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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >THRUST REVERSAL BY TUBULAR MASTIGONEMES - IMMUNOLOGICAL EVIDENCE FOR A ROLE OF MASTIGONEMES IN FORWARD MOTION OF ZOOSPORES OF PHYTOPHTHORA CINNAMOMI
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THRUST REVERSAL BY TUBULAR MASTIGONEMES - IMMUNOLOGICAL EVIDENCE FOR A ROLE OF MASTIGONEMES IN FORWARD MOTION OF ZOOSPORES OF PHYTOPHTHORA CINNAMOMI

机译：管状乳腺逆境的推力逆转-免疫学证据对乳腺激素在褐飞虱拟变的向前运动中的作用。

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摘要

The role of tubular mastigonemes in the reversal of thrust of the anterior flagellum of Phytophthora cinnamomi was analysed using mastigoneme-specific monoclonal antibodies and immunofluorescence and video microscopy. Exposure of live zoospores of P. cinnamomi to the mastigoneme-specific Zg antibodies caused alterations in the arrangement of mastigonemes on the flagellar surface and at Zg concentrations above 0.3 mu g/ml, mastigonemes became detached from the flagellum. As a consequence of antibody binding to the mastigonemes there were concentration-dependent perturbations in zoospore swimming behaviour and anterior flagellum beat pattern. With increasing antibody concentration zoospores swam more slowly and other parameters of their swimming pattern, such as the wavelength of the swimming helix and the frequency of rotation, were also reduced. The effects of Zg antibodies were specific at two levels: control immunoglobulins or antibodies that bound to other flagellar surface components did not have an effect on motility, and Zg antibodies did not interfere with the motility of zoospores of oomycete species to which they did not bind. The effects of antibody-induced disruption of mastigoneme arrangement strongly support previous hypotheses that tubular mastigonemes are responsible for thrust reversal by the anterior flagellum, enabling it to pull the cell through the surrounding medium. [References: 31]
机译：管状乳突线虫在逆疫霉菌鞭毛前推力逆转中的作用已使用乳突线虫特异的单克隆抗体，免疫荧光和视频显微镜进行了分析。肉桂假单胞菌的活的游动孢子暴露于鞭毛虫特异的Zg抗体会导致鞭毛表面上鞭毛虫的排列方式发生变化，并且当Zg浓度超过0.3μg / ml时，鞭毛虫会脱离鞭毛。由于抗体与乳突虫结合，因此在游动孢子的游泳行为和前鞭毛节拍模式中存在浓度依赖性的扰动。随着抗体浓度的增加，游动孢子游动更慢，并且其游动模式的其他参数（例如游动螺旋的波长和旋转频率）也降低了。 Zg抗体在两个水平上具有特异性：对照免疫球蛋白或与其他鞭毛表面成分结合的抗体对运动性没有影响，Zg抗体不会干扰不与之结合的卵菌种游动孢子的运动性。抗体诱导的乳突虫排列的破坏作用强烈支持先前的假设，即管状乳突虫负责前鞭毛逆转推力，使它能够将细胞拉过周围的培养基。 [参考：31]

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