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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >Morpho-anatomical characterization of mature embryo-derived callus of rice (Oryza sativa L.) suitable for transformation
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Morpho-anatomical characterization of mature embryo-derived callus of rice (Oryza sativa L.) suitable for transformation

机译：适用于转化的成熟胚衍生水稻（Oryza sativa L.）的形态解剖学表征

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The objective of this study was to morphoanatomically characterize embryogenic rice calli during early induction of somatic embryogenesis of three Brazilian rice cultivars. Herein, we explored embryogenic units (EUs) from 2-week-old cut proliferated calli to verify whether they were suitable for Agrobacterium tumefasciens-mediated transformation. Histological analysis and scanning electron microscopy (SEM) were used to analyze these types of calli during early rice callogenesis in the cultivars BRS Primavera, BRS Bonan?a, and BRS Caiapó. The characteristics of the embryogenic cells were preserved in the EUs, which showed a globular, compact structure that contained tightly packed cells and thus rendered the cells suitable for transformation. The EUs of BRS Caiapó also maintained the characteristics of the nonembryogenic callus, such as an elongated morphology and a lack of cellular organization. In general, the observations of the histological sections corresponded with those of the SEM images. The histological analysis suggested that all cultivars used in these experiments have morphogenic potential. The EUs from proliferated 2-week-old cut calli maintained their embryogenic features. The EUs were subjected to Agrobacterium-mediated transformation, which exhibited a regeneration frequency of 58 % for transformed hygromycin-resistant cell lines. These results show that EUs from proliferated 2-week-old cut calli are suitable for plant transformation.
机译：这项研究的目的是在三个巴西水稻品种的体细胞胚发生的早期诱导过程中，通过形态解剖学表征胚性水稻愈伤组织。在这里，我们探索了2周龄切开的增殖愈伤组织的胚发生单位（EUs），以验证它们是否适合于根癌农杆菌介导的转化。组织学分析和扫描电子显微镜（SEM）用于分析BRS Primavera，BRS Bonan？a和BRSCaiapó品种早稻愈伤过程中的这些类型的愈伤组织。胚胎发生细胞的特征保留在EU中，该细胞显示出球形紧密结构，其中包含紧密堆积的细胞，因此使这些细胞适合转化。 BRSCaiapó的EU也保留了非胚性愈伤组织的特征，例如形态延长和缺乏细胞组织。通常，组织切片的观察与SEM图像的观察相对应。组织学分析表明，这些实验中使用的所有品种均具有形态发生潜力。 2周龄切开的愈伤组织增殖的EU保持了其胚胎发生特征。 EU受到农杆菌介导的转化，对于转化的潮霉素抗性细胞系，其再生频率为58％。这些结果表明，来自增殖了两周龄的愈伤组织的EUs适合植物转化。

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