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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >A survey of cellulose microfibril patterns in dividing, expanding, and differentiating cells of Arabidopsis thaliana
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A survey of cellulose microfibril patterns in dividing, expanding, and differentiating cells of Arabidopsis thaliana

机译：拟南芥细胞分裂，扩增和分化过程中纤维素微纤维模式的研究

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摘要

Cellulose microfibrils are critical for plant cell specialization and function. Recent advances in live cell imaging of fluorescently tagged cellulose synthases to track cellulose synthesis have greatly advanced our understanding of cellulose biosynthesis. Nevertheless, cellulose deposition patterns remain poorly described inmany cell types, including those in the process of division or differentiation. In this study, we used field emission scanning electron microscopy analysis of cryo-planed tissues to determine the arrangement of cellulose microfibrils in various faces of cells undergoing cytokinesis or specialized development, including cell types in which cellulose cannot be imaged by conventional approaches. In dividing cells, we detected microfibrillar meshworks in the cell plates, consistent with the concentration at the cell plate of cellulose synthase complexes, as detected by fluorescently tagged CesA6. We also observed a loss of parallel cellulose microfibril orientation in walls of the mother cell during cytokinesis, which corresponded with the loss of fluorescently tagged cellulose synthase complexes from these surfaces. In recently formed guard cells, microfibrils were randomly organized and only formed a highly ordered circumferential pattern after pore formation. In pit fields, cellulose microfibrils were arranged in circular patterns around plasmodesmata. Microfibrils were random in most cotyledon cells except the epidermis and were parallel to the growth axis in trichomes. Deposition of cellulose microfibrils was spatially delineated in metaxylem and protoxylem cells of the inflorescence stem, supporting recent studies on microtubule exclusion mechanisms.
机译：纤维素微纤维对植物细胞的专业化和功能至关重要。荧光标记纤维素合成酶在活细胞成像中追踪纤维素合成的最新进展极大地提高了我们对纤维素生物合成的理解。然而，纤维素沉积模式仍然很难描述许多细胞类型，包括分裂或分化过程中的那些。在这项研究中，我们使用了冷冻平面组织的场发射扫描电子显微镜分析法来确定纤维素微纤维在经历胞质分裂或专门发育的细胞的各个面上的排列，包括无法通过常规方法对纤维素成像的细胞类型。在分裂的细胞中，我们检测到了细胞板中的微原纤维网，这与通过荧光标记的CesA6检测到的纤维素合酶复合物在细胞板上的浓度一致。我们还观察到胞质分裂过程中，母细胞壁中平行纤维素微纤维取向的丧失，这与这些表面上荧光标记的纤维素合酶复合物的丧失相对应。在最近形成的保卫细胞中，微纤维是随机组织的，并且在孔形成后仅形成高度有序的圆周图案。在坑田中，纤维素微纤维以圆形图案排列在胞质周围。除表皮外，大多数子叶细胞中的微纤维都是随机的，并且平行于毛状体的生长轴。纤维素微纤维的沉积在花序茎的后生木质部和protoxylem细胞中进行了空间划分，支持了对微管排斥机制的最新研究。

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