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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >The effect of LDL particles on the behaviour of epithelial noncancer and cancer cell lines after in vitro induced injury
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The effect of LDL particles on the behaviour of epithelial noncancer and cancer cell lines after in vitro induced injury

机译：LDL颗粒对体外诱导损伤后上皮非癌细胞和癌细胞系行为的影响

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摘要

Cell spreading capability and cell proliferation are the major processes in wound healing of injured epithelia as well as in tumour progression. The effect of low-density lipoprotein (LDL) particles as a major extracellular source of cholesterol was evaluated in the re-epithelisation assay of in vitro induced injury. We selected two noncancer cell lines with different dependence on LDL concentrations, the kidney epithelial cells (MDCK) with higher dependence and keratinocytes (HaCaT) with lower dependence on LDL, and three cancer cell lines originating from epithelial cells: A549 (alveolar), CaCo-2 (intestinal) and RT4 (urothelial). All cells were incubated in a control medium, in an LDL-enriched medium or in an LDL-deficient medium. The LDL-enriched medium stimulated cell spreading of MDCK cells which, together with increased proliferation of these cells, resulted in an enhanced re-epithelisation of in vitro induced injury. LDL deficiency caused lower cell spreading which resulted in a decreased re-epithelisation despite the higher proliferation of MDCK cells in this medium. The re-epithelisation of keratinocytes (HaCaT) was not affected by altered LDL concentrations. In cancer cell lines A549, CaCo-2 and RT4, wide heterogeneity regarding cell proliferation and spreading capability was observed after treatment with different LDL concentrations. LDL had no influence on actin filament and tight junction distribution in any of the tested cell lines. The cholesterol content of all cell types, except for CaCo-2 cells, proved to be independent of the LDL level. Further research of the beneficial effects of LDL is needed to prove LDL as a safe enhancer of epithelial wound healing.
机译：细胞扩散能力和细胞增殖是受伤的上皮的伤口愈合以及肿瘤进展的主要过程。低密度脂蛋白（LDL）颗粒作为胆固醇的主要细胞外来源的作用在体外诱导损伤的重新上皮化测定中进行了评估。我们选择了两种对LDL浓度依赖性不同的非癌细胞系，对LDL依赖性较高的肾上皮细胞（MDCK）和对LDL依赖性较低的角质形成细胞（HaCaT），以及三种源自上皮细胞的癌细胞系：A549（肺泡），CaCo -2（肠）和RT4（尿路上皮）。将所有细胞在对照培养基，富含LDL的培养基或缺乏LDL的培养基中孵育。富含LDL的培养基刺激了MDCK细胞的细胞扩散，其与这些细胞的增殖增加一起，导致体外诱导的损伤的上皮再增强。 LDL缺乏导致较低的细胞扩散，尽管MDCK细胞在该培养基中有较高的增殖，但导致上皮减少。 LDL浓度改变不会影响角质形成细胞（HaCaT）的重新上皮。在癌细胞系A549，CaCo-2和RT4中，用不同的LDL浓度处理后，观察到关于细胞增殖和扩散能力的广泛异质性。 LDL对任何测试的细胞系中的肌动蛋白丝和紧密连接分布没有影响。除了CaCo-2细胞外，所有其他细胞类型的胆固醇含量均与LDL水平无关。需要进一步研究LDL的有益作用，以证明LDL是上皮伤口愈合的安全增强剂。

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