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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >Trehalose pretreatment induces salt tolerance in rice (Oryza sativa L.) seedlings: oxidative damage and co-induction of antioxidant defense and glyoxalase systems
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Trehalose pretreatment induces salt tolerance in rice (Oryza sativa L.) seedlings: oxidative damage and co-induction of antioxidant defense and glyoxalase systems

机译：海藻糖预处理诱导水稻（Oryza sativa L.）幼苗的耐盐性：氧化损伤以及抗氧化防御和乙二醛酶系统的共诱导

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Salinity in the form of abiotic stress adversely effects plant growth, development, and productivity. Various osmoprotectants are involved in regulating plant responses to salinity; however, the precise role of trehalose (Tre) in this process remains to be further elucidated. The present study investigated the regulatory role of Tre in alleviating salt-induced oxidative stress in hydroponically grown rice seedlings. Salt stress (150 and 250 mM NaCl) for 72 h resulted in toxicity symptoms such as stunted growth, severe yellowing, and leaf rolling, particularly at 250 mM NaCl. Histochemical observation of reactive oxygen species (ROS; O-2 (a (TM) a') and H2O2) indicated evident oxidative stress in salt-stressed seedlings. In these seedlings, the levels of lipoxygenase (LOX) activity, malondialdehyde (MDA), H2O2, and proline (Pro) increased significantly whereas total chlorophyll (Chl) and relative water content (RWC) decreased. Salt stress caused an imbalance in non-enzymatic antioxidants, i.e., ascorbic acid (AsA) content, AsA/DHA ratio, and GSH/GSSG ratio decreased but glutathione (GSH) content increased significantly. In contrast, Tre pretreatment (10 mM, 48 h) significantly addressed salt-induced toxicity symptoms and dramatically depressed LOX activity, ROS, MDA, and Pro accumulation whereas AsA, GSH, RWC, Chl contents, and redox status improved considerably. Salt stress stimulated the activities of SOD, GPX, APX, MDHAR, DHAR, and GR but decreased the activities of CAT and GST. However, Tre-pretreated salt-stressed seedlings counteracted SOD and MDHAR activities, elevated CAT and GST activities, further enhanced APX and DHAR activities, and maintained GPX and GR activities similar to the seedlings stressed with salt alone. In addition, Tre pretreatment enhanced the activities of methylglyoxal detoxifying enzymes (Gly I and Gly II) more efficiently in salt-stressed seedlings. Our results suggest a role for Tre in protecting against salt-induced oxidative damage attributed to reduced ROS accumulation, elevation of non-enzymatic antioxidants, and co-activation of the antioxidative and glyoxalase systems.
机译：非生物胁迫形式的盐度会对植物的生长，发育和生产力产生不利影响。各种渗透保护剂都参与调节植物对盐分的反应。然而，海藻糖（Tre）在该过程中的确切作用还有待进一步阐明。本研究调查了Tre在缓解水培水稻幼苗中盐诱导的氧化胁迫中的调节作用。持续72小时的盐胁迫（150和250 mM NaCl）会导致毒性症状，例如生长发育迟缓，严重发黄和卷叶，特别是在250 mM NaCl下。活性氧（ROS; O-2（TM）a'）和H2O2的组织化学观察表明，盐胁迫幼苗中存在明显的氧化应激。在这些幼苗中，脂氧合酶（LOX）活性，丙二醛（MDA），H2O2和脯氨酸（Pro）的水平显着增加，而总叶绿素（Chl）和相对含水量（RWC）降低。盐胁迫导致非酶抗氧化剂的不平衡，即抗坏血酸（AsA）含量，AsA / DHA比和GSH / GSSG比降低，但谷胱甘肽（GSH）含量显着增加。相反，Tre预处理（10 mM，48小时）显着解决了盐诱导的毒性症状，并显着降低了LOX活性，ROS，MDA和Pro积累，而AsA，GSH，RWC，Chl含量和氧化还原状态则显着改善。盐胁迫刺激了SOD，GPX，APX，MDHAR，DHAR和GR的活性，但降低了CAT和GST的活性。然而，Tre预处理的盐胁迫幼苗抵消了SOD和MDHAR活性，CAT和GST活性升高，进一步增强了APX和DHAR活性，并保持了GPX和GR活性，类似于仅用盐胁迫的幼苗。此外，Tre预处理在盐胁迫的幼苗中更有效地增强了甲基乙二醛解毒酶（Gly I和Gly II）的活性。我们的结果表明，Tre在防止盐引起的氧化损伤中起着一定的作用，归因于ROS的减少，非酶类抗氧化剂的升高以及抗氧化和乙二醛酶系统的共活化。

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