...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >Searching chromosomal landmarks in Indian lentils through EMA-based Giemsa staining method
【24h】

Searching chromosomal landmarks in Indian lentils through EMA-based Giemsa staining method

机译：通过基于EMA的吉姆萨染色方法搜索印度小扁豆中的染色体标志物

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Lentil is one of the oldest protein-rich food crop with only one cultivated and six wild species. India is one important cultivator, producer and consumer of lentils and possesses a large number of germplasms. All species of lentil show 2n = 14 chromosomes. The primary objective of the present paper is to search chromosomal landmarks through enzymatic maceration and air drying (EMA)-based Giemsa staining method in five Indian lentil species not reported elsewhere at a time. Additionally, gametic chromosome analysis, tendril formation and seed morphology have been studied to ascertain interspecific relationships in lentils. Chromosome analysis in Lens culinaris, Lens orientalis and Lens odemensis revealed that they contain intercalary sat chromosome and similar karyotypic formula, while Lens nigricans and Lens lamottei showed presence of terminal sat chromosomes not reported earlier. This distinct morphological feature in L. nigricans and L. lamottei may be considered as chromosomal landmark. Meiotic analysis showed n = 7 bivalents in L. culinaris, L. nigricans and L. lamottei. No tendril formation was observed in L. culinaris, L. orientalis and L. odemensis while L. nigricans and L. lamottei developed very prominent tendrils. Based on chromosomal analysis, tendril formation and seed morphology, the five lentil species can be separated into two distinct groups. The outcome of this research may enrich conventional and biotechnological breeding programmes in lentil and may facilitate an easy and alternative method for identification of interspecific hybrids.
机译：扁豆是最古老的富含蛋白质的粮食作物之一，只有一种栽培种和六种野生种。印度是小扁豆的重要栽培者，生产者和消费者，并且拥有大量种质。所有小扁豆均显示2n = 14条染色体。本文的主要目的是通过基于酶浸解和风干（EMA）的Giemsa染色方法搜索五个印度小扁豆物种的染色体标志物，这些物种一次未在其他地方报道。此外，已经进行了配子染色体分析，卷须形成和种子形态研究，以确定小扁豆中的种间关系。 Lens culinaris，Lens Orientalis和Lens odemensis的染色体分析表明，它们含有inter插染色体和类似的核型，而Ligs nigricans和Lens lamottei表明存在未见报道的末端sat染色体。黑黑麦草和拉美乳杆菌的这种独特的形态学特征可以被认为是染色体的标志。减数分裂分析显示在库氏乳杆菌，黑乳杆菌和拉美乳杆菌中n ＝ 7个二价。在culinaris，L.orientalis和odemensis中没有观察到卷须的形成，而nigricans和lamottei则形成了非常突出的卷须。根据染色体分析，卷须形成和种子形态，可以将5种小扁豆分为两个不同的组。这项研究的结果可能会丰富小扁豆的常规育种和生物技术育种计划，并可能有助于鉴定种间杂种的简便易行的方法。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号