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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >Oxidative and antioxidative responses in the wheat-Azospirillum brasilense interaction
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Oxidative and antioxidative responses in the wheat-Azospirillum brasilense interaction

机译：小麦-巴西螺旋藻相互作用中的氧化和抗氧化反应

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Azospirillum is a plant growth-promoting rhizobacteria (PGPR) able to enhance the growth of wheat. The aim of this study was to test the effect of Azospirillum brasilense cell wall components on superoxide (O-2(center dot-)) production in wheat roots and the effect of oxidative stress on A. brasilense viability. We found that inoculation with A. brasilense reduced O-2(center dot-) levels by approx. 30 % in wheat roots. Inoculation of wheat with papain-treated A. brasilense, a Cys protease, notably increased O-2(center dot-) production in all root tissues, as was observed by the nitro blue tetrazolium (NBT) reduction. However, a 24-h treatment with rhizobacteria lipopolysaccharides (50 and 100 mu g/mL) alone did not affect the pattern of O-2(center dot-) production. Analysis of the effect of plant cell wall components on A. brasilense oxidative enzyme activity showed no changes in catalase activity but a decrease in superoxide dismutase activity in response to polygalacturonic acid treatment. Furthermore, A. brasilense growth was only affected by high concentrations of H2O2 or paraquat, but not by sodium nitroprusside. Our results suggest that rhizobacterial cell wall components play an important role in controlling plant cell responses and developing tolerance of A. brasilense to oxidative stress produced by the plant.
机译：固氮螺菌是一种能够促进小麦生长的植物促生根瘤菌（PGPR）。这项研究的目的是测试巴西固氮螺旋菌细胞壁成分对小麦根中超氧化物（O-2（中心点））产生的影响以及氧化胁迫对巴西柔顺菌生存力的影响。我们发现用巴西拟南芥接种可降低O-2（中心点）水平。小麦根中占30％。用木瓜蛋白酶处理过的巴西青霉（一种半胱氨酸蛋白酶）接种小麦，可显着增加所有根组织中O-2（中心点）的产量，这可通过硝基蓝四唑（NBT）的还原来观察。但是，仅用根际细菌脂多糖（50和100μg / mL）进行24小时处理不会影响O-2（中心点）的产生。分析植物细胞壁成分对巴西布鲁氏菌氧化酶活性的影响表明，响应于聚半乳糖醛酸处理，过氧化氢酶活性无变化，但超氧化物歧化酶活性下降。此外，巴西柔菇的生长仅受高浓度的H2O2或百草枯的影响，而不受硝普钠的影响。我们的结果表明，根瘤菌细胞壁成分在控制植物细胞反应和发展巴西农杆菌对植物产生的氧化胁迫的耐受性中起重要作用。

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