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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >The solute specificity profiles of nucleobase cation symporter 1 (NCS1) from Zea mays and Setaria viridis illustrate functional flexibility
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The solute specificity profiles of nucleobase cation symporter 1 (NCS1) from Zea mays and Setaria viridis illustrate functional flexibility

机译：玉米和狗尾草中核碱基阳离子共转运蛋白1（NCS1）的溶质特异性谱说明功能灵活性

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摘要

The solute specificity profiles (transport and binding) for the nucleobase cation symporter 1 (NCS1) proteins, from the closely related C4 grasses Zea mays and Setaria viridis, differ from that of Arabidopsis thaliana and Chlamydomonas reinhardtii NCS1. Solute specificity profiles for NCS1 from Z. mays (ZmNCS1) and S. viridis (SvNCS1) were determined through heterologous complementation studies in NCS1-deficient Saccharomyces cerevisiae strains. The four Viridiplantae NCS1 proteins transport the purines adenine and guanine, but unlike the dicot and algal NCS1, grass NCS1 proteins fail to transport the pyrimidine uracil. Despite the high level of amino acid sequence similarity, ZmNCS1 and SvNCS1 display distinct solute transport and recognition profiles. SvNCS1 transports adenine, guanine, hypoxanthine, cytosine, and allantoin and competitively binds xanthine and uric acid. ZmNCS1 transports adenine, guanine, and cytosine and competitively binds, 5-fluorocytosine, hypoxanthine, xanthine, and uric acid. The differences in grass NCS1 profiles are due to a limited number of amino acid alterations. These amino acid residues do not correspond to amino acids essential for overall solute and cation binding or solute transport, as previously identified in bacterial and fungal NCS1, but rather may represent residues involved in subtle solute discrimination. The data presented here reveal that within Viridiplantae, NCS1 proteins transport a broad range of nucleobase compounds and that the solute specificity profile varies with species.
机译：来自紧密相关的C4草类玉米和狗尾草的核碱基阳离子同向转运蛋白1（NCS1）蛋白的溶质特异性谱（转运和结合）不同于拟南芥和莱茵衣藻NCS1。通过异源互补研究在NCS1基因缺陷型酿酒酵母菌株中确定了玉米（ZmNCS1）和绿色链球菌（SvNCS1）NCS1的溶质特异性谱。四种Vi科植物NCS1蛋白可转运嘌呤腺嘌呤和鸟嘌呤，但与双子叶植物和藻类NCS1不同，草类NCS1蛋白无法转运嘧啶尿嘧啶。尽管氨基酸序列相似性很高，ZmNCS1和SvNCS1仍显示出不同的溶质转运和识别特性。 SvNCS1转运腺嘌呤，鸟嘌呤，次黄嘌呤，胞嘧啶和尿囊素，并竞争性结合黄嘌呤和尿酸。 ZmNCS1转运腺嘌呤，鸟嘌呤和胞嘧啶，并竞争性结合5-氟胞嘧啶，次黄嘌呤，黄嘌呤和尿酸。草NCS1配置文件的差异是由于氨基酸改变的数量有限。如先前在细菌和真菌NCS1中鉴定的，这些氨基酸残基不对应于整体溶质和阳离子结合或溶质运输所必需的氨基酸，而是可能代表参与微妙溶质识别的残基。此处提供的数据表明，在Viridiplantae中，NCS1蛋白可转运各种核碱基化合物，并且溶质特异性谱随物种而变化。

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