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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >CELL SURFACE ARABINOGALACTAN-PROTEINS AND THEIR RELATION TO CELL PROLIFERATION AND VIABILITY
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CELL SURFACE ARABINOGALACTAN-PROTEINS AND THEIR RELATION TO CELL PROLIFERATION AND VIABILITY

机译：细胞表面阿拉伯半乳聚糖蛋白及其与细胞增殖和存活率的关系

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Two ''Paul's Scarlet'' rose (Rosa sp.) suspension cultures, Rosa 57 and Rosa 93, differed in their response to (beta-D-Glc)(3), a Yariv phenylglycoside that selectively binds to arabinogalactan-proteins (AGPs). The growth of both of these cell lines was strongly inhibited by (beta-D-Glc)(3), but while the effect was readily reversible with Rosa 57, initial tests showed that the viability of Rosa 93 was severely compromised. Over a period of time, as the cell cultures were passaged in normal medium, aliquots were drawn and tested for sensitivity to (beta-D-Glc)(3). While all tests of Rosa 93 continued to show that (beta-D-Glc)(3) strongly inhibited growth, the viability and capacity for growth recovery after treatment progressively improved with passage number. The decreased proliferation and/or viability of these cell cultures in the presence of (beta-D-Glc)(3) was hypothesized to be due to the perturbation of AGPs. Since Rosa 57 and Rosa 93 differ in their response to (beta-D-Glc)(3), this hypothesis led to the prediction that these cell lines would also differ in their AGP complements. Electrophoretic and compositional analyses showed that Rosa 57 and Rosa 93 did differ in both the numbers and abundances of AGPs isolated from the cell walls and the conditioned culture medium. The amount of cell wall-associated AGPs in Rosa 93 increased with passage number and reached approximately three times the amount of cell wall-associated AGPs in Rosa 57. Conversely, Rosa 57 suspension cells accumulated a greater amount of AGPs in their conditioned culture medium than did Rosa 93 suspension cells. A cell cycle analysis was performed on Rosa 57 cells that were treated with (beta-D-Glc)(3) and blocked in cell division. The results showed that the cells were not preferentially arrested in S, G(2), or M phase. [References: 44]
机译：两种``Paul's Scarlet''玫瑰（Rosa sp。）悬浮培养物Rosa 57和Rosa 93对（beta-D-Glc）（3）（一种选择性结合阿拉伯半乳聚糖蛋白（AGP）的Yariv苯基糖苷）的反应有所不同）。这两种细胞系的生长都受到（β-D-Glc）（3）的强烈抑制，但是当使用Rosa 57可以轻松逆转这种作用时，初步测试表明Rosa 93的生存能力受到了严重损害。在一段时间内，随着细胞培养物在正常培养基中传代，抽取等分试样并测试对（β-D-Glc）（3）的敏感性。尽管Rosa 93的所有测试均继续显示（β-D-Glc）（3）强烈抑制生长，但处理后的存活力和生长恢复能力随着传代次数的增加而逐渐提高。假定存在（β-D-Glc）（3）时，这些细胞培养物的增殖和/或活力降低，这是由于AGP的扰动引起的。由于Rosa 57和Rosa 93对（beta-D-Glc）（3）的反应不同，因此该假设导致了以下预测：这些细胞系的AGP补体也将有所不同。电泳和成分分析表明，从细胞壁和条件培养基分离出的AGP的数量和丰度，Rosa 57和Rosa 93确实存在差异。 Rosa 93中与细胞壁相关的AGP的数量随传代次数的增加而增加，并达到Rosa 57中与细胞壁相关的AGP数量的大约三倍。相反，Rosa 57悬浮细胞在其条件培养基中积累的AGP数量大于罗莎93悬浮细胞。对经（β-D-Glc）（3）处理并在细胞分裂中受阻的Rosa 57细胞进行了细胞周期分析。结果表明，细胞没有优先停滞在S，G（2）或M期。 [参考：44]

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