Optimizing conditions for tobacco BY-2 cell cycle synchronization - Rapid communication

Samuels AL.; Lipe M.; Staehelin LA.; Meehl J.

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Optimizing conditions for tobacco BY-2 cell cycle synchronization - Rapid communication

机译：烟草BY-2细胞周期同步的优化条件-快速交流

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Tobacco BY-2 cells have become. a major tool in plant cell biological research, in part due to the availability of a cell cycle synchronization protocol. This method, pioneered by Nagata and coworkers, involves sequential treatments with aphidicolin (a DNA synthesis inhibitor) and propyzamide (a microtubule inhibitor which arrests mitosis). The effects of these inhibitors are reversible, allowing the cell culture to progress into M phase synchronously. However, attempts to reproduce high levels of synchrony with published protocols have not been uniformly successful. This paper describes critical parameters for cell cycle synchronization and documents the kinetics and variation typically found in using this protocol. [References: 14]

机译：烟草已成为BY-2细胞。植物细胞生物学研究中的主要工具，部分归因于细胞周期同步协议的可用性。这种方法是由永田（Nagata）及其同事首创的，涉及用蚜虫药（一种DNA合成抑制剂）和丙酰胺（一种阻止有丝分裂的微管抑制剂）相继治疗。这些抑制剂的作用是可逆的，使细胞培养同步地进入M期。但是，尝试重现与已发布协议的高度同步并没有取得统一的成功。本文介绍了细胞周期同步的关键参数，并记录了使用此协议时通常发现的动力学和变化。 [参考：14]

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《Protoplasma: An International Journal of Cell Biology》 |1998年第4期|共5页
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Samuels AL.; Lipe M.; Staehelin LA.; Meehl J.;
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正文语种 eng
中图分类细胞形态学;
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1. Optimizing conditions for tobacco BY-2 cell cycle synchronization - Rapid communication [J] . Samuels AL., Lipe M., Staehelin LA., Protoplasma: An International Journal of Cell Biology . 1998,第3a4期

机译：烟草BY-2细胞周期同步的优化条件-快速交流
2. Cryptogein-Induced Cell Cycle Arrest at G(2) Phase is Associated with Inhibition of Cyclin-Dependent Kinases, Suppression of Expression of Cell Cycle-Related Genes and Protein Degradation in Synchronized Tobacco BY-2 Cells [J] . Ohno Ryoko, Kadota Yasuhiro, Fujii Shinsuke, Plant and cell physiology . 2011,第5期

机译：在G（2）期Cryptogein诱导的细胞周期阻滞与细胞周期蛋白依赖性激酶的抑制，细胞周期相关基因表达的抑制和同步烟草BY-2细胞蛋白降解相关。
3. Transient accumulation of cis- and trans-zeatin type cytokinins and its relation to cytokinin oxidase activity during cell cycle of synchronized tobacco BY-2 cells [J] . Dobrev P, Motyka V, Gaudinova A, Plant Physiology and Biochemistry . 2002,第4期

机译：同步烟草BY-2细胞在细胞周期中顺式和反式玉米素型细胞分裂素的瞬时积累及其与细胞分裂素氧化酶活性的关系
4. Study on Toxicity of Copper Oxide Engineered Nanoparticles to Tobacco BY-2 Cells [C] . Lili Xu, Haijun Sui, Xiaoyun Liu, International Conference on Mechanical Science and Engineering . 2013

机译：铜氧化铜工程纳米粒子毒性研究烟草 - 2细胞的毒性
5. Auxin deprivation induces Golgi stack differentiation in suspension-cultured tobacco BY-2 cells [D] . Winicur, Zev Michael 1997

机译：生长素剥夺在悬浮培养的烟草BY-2细胞中诱导高尔基体堆栈分化
6. Two Arabidopsis cyclin promoters mediate distinctive transcriptional oscillation in synchronized tobacco BY-2 cells. [O] . O Shaul, V Mironov, S Burssens, 1996

机译：两个拟南芥细胞周期蛋白启动子在同步烟草BY-2细胞中介导独特的转录振荡。
7. Levels of endogenous cytokinins, indole-3-acetic acid and abscisic acid during the cell cycle of synchronized tobacco BY-2 cells [O] . Redig Pascale, Shaul Orit, Inzé Dirk, 1996

机译：同步烟草BY-2细胞细胞周期中内源性细胞分裂素，吲哚-3-乙酸和脱落酸的水平
8. Synchronization. I. The Panel Chairman'S Introduction. Ii. Synchronization in Data Communication. Iii. On Synchronization in Communication Theory. Iv. Synchronization and Bit Timing. V. Word Synchronization Over Noisy Channels. [R] . Golomb, S. W., Davey, J. R., Reed, I. S., 1963

机译：同步。 I.小组主席的介绍。二。数据通信中的同步。三。论传播理论中的同步。四。同步和位定时。 V.噪声信道上的字同步。

Optimizing conditions for tobacco BY-2 cell cycle synchronization - Rapid communication

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