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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >Virus assembly in Hincksia hincksiae (Ectocarpales, Phaeophyceae) an electron and fluorescence microscopic study
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Virus assembly in Hincksia hincksiae (Ectocarpales, Phaeophyceae) an electron and fluorescence microscopic study

机译：电子学和荧光显微镜研究Hincksia hincksiae（Ectocarpales，Phaeophyceae）中的病毒装配

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The filamentous brown alga Hincksia hincksiae can be infected by a large icosahedral double-stranded DNA virus (HincV-1). The virus shows extended latency and is replicated only in cells homologous to sporangia. Virus formation was studied by transmission electron microscopy, DAPI staining, and beta-tubulin immunofluorescence. Inhibition of cytokineses results in multinucleate cells, which are the first indication of virus replication in productive cells; the microtubular cytoskeleton does nor seem to be affected by the virus. Replication of viral DNA begins in the nuclei, which increase in size and eventually disintegrate. Virus assembly takes place in a mixed nucleo-/cytoplasm. Capsids bud from cisternae, which are interpreted as modified endoplasmic reticulum aggregated to virus assembly centres. The internal membranous component of the virus is thus derived from the endoplasmic reticulum. The particles are empty (electron translucent) when assembled, and the nucleoprotein core seems to be packaged subsequently through an opening in the capsid. A number of fine structural features not previously reported from brown algae and related to virus formation are described. Our results on Hincksia hincksiae virus are compared with observations made on various other icosahedral DNA viruses infecting eukaryotic algae and animals. [References: 37]
机译：丝状褐藻Hincksia hincksiae可以被大型二十面体双链DNA病毒（HincV-1）感染。该病毒显示出延长的潜伏期，仅在与孢子囊同源的细胞中复制。通过透射电子显微镜，DAPI染色和β-微管蛋白免疫荧光研究了病毒的形成。抑制细胞因子会产生多核细胞，这是病毒在生产性细胞中复制的第一个迹象。微管细胞骨架似乎也不受病毒影响。病毒DNA的复制在细胞核中开始，其大小增加并最终分解。病毒组装发生在混合的核/细胞质中。衣壳从水箱中萌芽，这被解释为聚集到病毒装配中心的修饰的内质网。因此，病毒的内部膜成分源自内质网。组装时颗粒是空的（电子透明），核蛋白核似乎随后通过衣壳中的开口包装。描述了许多以前从未从褐藻中报道过并与病毒形成有关的精细结构特征。我们在Hincksia hincksiae病毒上的结果与感染真核藻类和动物的其他各种二十面体DNA病毒的观察结果进行了比较。 [参考：37]

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