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首页> 外文期刊>The Journal of Antibiotics: An International Journal >Identification of the biosynthetic gene cluster of A-500359s in Streptomyces griseus SANK60196.
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Identification of the biosynthetic gene cluster of A-500359s in Streptomyces griseus SANK60196.

机译：鉴定灰链霉菌SANK60196中A-500359s的生物合成基因簇。

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摘要

A-500359s, produced by Streptomyces griseus SANK60196, are inhibitors of bacterial phospho-N-acetylmuramyl-pentapeptide translocase. They are composed of three distinct moieties: a 5'-carbamoyl uridine, an unsaturated hexuronic acid and an aminocaprolactam. Two contiguous cosmids covering a 65-kb region of DNA and encoding 38 open reading frames (ORFs) putatively involved in the biosynthesis of A-500359s were identified. Reverse transcriptase PCR showed that most of the 38 ORFs are highly expressed during A-500359s production, but mutants that do not produce A-500359s did not express these same ORFs. Furthermore, orf21, encoding a putative aminoglycoside 3'-phosphotransferase, was heterologously expressed in Escherichia coli and Streptomyces albus, yielding strains having selective resistance against A-500359B, suggesting that ORF21 phosphorylates the unsaturated hexuronic acid as a mechanism of self-resistance to A-500359s. In total, the data suggest that the cloned region is involved in the resistance, regulation and biosynthesis of A-500359s.
机译：由灰链霉菌SANK60196生产的A-500359s是细菌磷酸-N-乙酰基村mura基-五肽转位酶的抑制剂。它们由三个不同的部分组成：5'-氨基甲酰基尿苷，不饱和己糖醛酸和氨基己内酰胺。确定了两个连续的粘粒，它们覆盖DNA的65 kb区域并编码38个开放阅读框（ORF），这些阅读框可能与A-500359s的生物合成有关。逆转录酶PCR显示38个ORF中的大多数在A-500359s产生期间高表达，但是不产生A-500359s的突变体不表达这些相同的ORF。此外，编码假定的氨基糖苷3'-磷酸转移酶的orf21在大肠杆菌和白色链霉菌中异源表达，产生对A-500359B具有选择性抗性的菌株，表明ORF21将不饱和己糖醛酸磷酸化为对A的自抗性机制-500359s。总体而言，数据表明克隆的区域参与了A-500359s的抗性，调控和生物合成。

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