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首页> 外文期刊>The Journal of Antibiotics: An International Journal >Collinone,a New Recombinant Angular Polyketide antibiotic Made by an Engineered Streptomyces Strain
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Collinone,a New Recombinant Angular Polyketide antibiotic Made by an Engineered Streptomyces Strain

机译：通过工程链霉菌菌株生产的新型重组角聚酮类抗生素科利农

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Large chromosomal DNA fragments containing different parts of the putative rubromycin polyketide synthase gene cluster were cloned and functionaliy expressed in S. coelicolor CH999. Expression of these clones yielded 5~10 metabolites that were not detected in S. collinus culture extracts. This paper focusses on 9ne of the new metabolites, termed collinone, that was isolated in large quantities and purified for spectroscopic structure determination and biological screening assays. Collinone is a heavily oxidized angular hexacyclic compound containing an unusuall,4,5,8(2H,3H)-anthracenetetrone moiety previously only reported to be present in antibiotics SF2446A1., A2, A3, Bl and B2 isolated from Streptomyces sp. SF2446. Structure analysis of collinone indicates a tridecaketide with a 26 carbon backbone. The basic benz[a]naphthacene ring system of collinone is angular, similar to the aglycones of the well- known angucycline and angucyclinone antibiotics. While collinone showed antibacterial activity against vancomycin-resistant enterococci, no antifungal or significant antiviral activities were detected. Collinone could be a good starting point to obtain new bioactive angucyclin(on)e-like compounds by further genetic engineering of its pathway.
机译：克隆了包含推定的红霉素聚酮化合物合酶基因簇的不同部分的大染色体DNA片段，并在大肠杆菌中进行了功能表达。这些克隆的表达产生了5〜10种代谢产物，在Collinus培养物中未检测到。本文重点研究了9ne的新代谢物，称为可乐酮，其被大量分离并纯化用于光谱结构测定和生物筛选测定。 Collinone是一种重度氧化的角六环化合物，含有一个不寻常的4,4,5,8（2H，3H）-蒽酮酮部分，以前仅据报道存在于从链霉菌属种分离的抗生素SF2446A1，A2，A3，B1和B2中。 SF2446。科林酮的结构分析表明具有26个碳主链的三蝶烯。可乐酮的基本苯并[a]萘并环体系是有角的，类似于众所周知的an环素和an环素类抗生素的糖苷配基。虽然可乐酮对耐万古霉素的肠球菌具有抗菌活性，但未检测到抗真菌或重要的抗病毒活性。通过对其途径进行进一步的基因工程改造，Collinone可能是获得新的具有生物活性的angucyclin（on）e样化合物的良好起点。

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