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首页> 外文期刊>The Journal of Antibiotics: An International Journal >Effects of Aflastatin A,an Inhibitor of Aflatoxin Production,on Aflatoxin Biosynthetic Pathway and Glucose Metabolismin Aspergillus parasiticus
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Effects of Aflastatin A,an Inhibitor of Aflatoxin Production,on Aflatoxin Biosynthetic Pathway and Glucose Metabolismin Aspergillus parasiticus

机译：黄曲霉毒素生产抑制剂黄曲霉毒素A对寄生曲霉黄曲霉毒素生物合成途径和葡萄糖代谢的影响

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摘要

Aflastatin A inhibits aflatoxin production by Aspergillus parasiticus via an unknown mechanism.We found that aflastatin A clearly inhibited production of norsolorinic acid,an early biosynthetic intermediate of aflatoxin,at a concentration of 0.25 #mu#g/ml.Reversetranscriptase polymerase chain reaction (RT-PCR),and real-time quantitative PCR (TaqMan PCR)experiments indicated tat the transcription of genes encoding aflatoxin biosynthetic enzymes (pksA,ver-1,and omtA) and a gene encoding a regulatory protein forexpression of the biosynthetic enzymes (aflR) were significantly reduced by the addition of aflastatin A.We also found that aflastatin A elevated the glucose consumption and ethanol accumulation by A.parasiticus,and repressed transcription of genes involved in ethanol utilization.These results suggest that aflastatin A inhibits a very early step in aflatoxin biosynthesis prior to the transcription of aflR and can influence glucose metabolismin the fungus.
机译：黄素抑制素A通过未知的机制抑制寄生曲霉产生黄曲霉毒素。我们发现黄素抑制素A以0.25＃μ＃g / ml的浓度明显抑制了黄曲霉毒素的早期生物合成中间体降冰片酸的生成。逆转录聚合酶链反应（RT） -PCR）和实时定量PCR（TaqMan PCR）实验表明，编码黄曲霉毒素生物合成酶（pksA，ver-1和omtA）的基因和编码调节蛋白以表达生物合成酶（aflR）的基因的转录加入黄素他汀A可以显着降低黄曲霉素A的含量。我们还发现黄素他汀A可以提高寄生虫曲霉的葡萄糖消耗和乙醇积累，并抑制参与乙醇利用的基因的转录。黄曲霉毒素在aflR转录之前进行生物合成，并且可以影响真菌中的葡萄糖代谢。

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