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首页> 外文期刊>The Journal of Antibiotics: An International Journal >Selective production of fungal beauveriolide I or III by fermentation in amino acid-supplemented media
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Selective production of fungal beauveriolide I or III by fermentation in amino acid-supplemented media

机译：通过在氨基酸补充培养基中发酵选择性生成真菌性金紫苏内酯I或III

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摘要

Beauveriolides I and III,cyclic depsipeptides composed of L-Phe,L-Ala,D-Leu and (3S,4S)-3-hydroxy-4-methyloctanoic acid,and L-Phe,L-Allo-Ile,L-allo-Ile and (3S,4S)-3-hydroxy-4-methyloctanoic acid,respectively,wee previously isolated from the culture broth of fungal Beauveria sp.FO-6979 as inhibitors of macrophage foam cell formation.To improve the production of these compoudns by fermentation,the culture conditions were studied.The production of both beauveriolides was increased five to tenfolds by fermentation in the culture media containing tryptone.Further study revealed that addition of L-Leu/l-Ile,but not D-Leu/D-allo-Ile,to the culture medium yielded a high and selective production of beauveriolide I or III.As a result,regardless of their separation difficulty due to the similar physico-chemical properties,a large amount of beauveriolide I or III was prepared from the culture broth obtained from L-Leu-or L-Ile-supplemented fermentation,respectivley,by one step purification using silica gel column chromatography.
机译：白僵菌内酯I和III，由L-Phe，L-Ala，D-Leu和（3S，4S）-3-羟基-4-甲基辛酸和L-Phe，L-Allo-Ile，L-allo组成的环状二肽-Ile和（3S，4S）-3-羟基-4-甲基辛酸分别从真菌白僵菌FO-6979的培养液中分离出来，作为巨噬细胞泡沫细胞形成的抑制剂。为提高这些化合物的产量通过发酵，研究了培养条件。在含胰蛋白p的培养基中发酵，两种金紫苏内酯的产量增加了五到十倍。因此，无论由于相似的理化性质而导致分离困难，都可以从培养基中异丁烯中高选择性地生产出贝佛洛利德I或III。从L-Leu-或L-Ile补充的发酵中获得的培养液，分别通过一步纯化硅胶柱色谱。

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