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首页> 外文期刊>The Journal of Antibiotics: An International Journal >Biosynthetic potential of sesquiterpene synthases: product profiles of Egyptian Henbane premnaspirodiene synthase and related mutants
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Biosynthetic potential of sesquiterpene synthases: product profiles of Egyptian Henbane premnaspirodiene synthase and related mutants

机译：倍半萜合酶的生物合成潜力：埃及Henbane premnaspirodiene合酶和相关突变体的产品概况

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摘要

The plant terpene synthase (TPS) family is responsible for the biosynthesis of a variety of terpenoid natural products possessing diverse biological functions. TPSs catalyze the ionization and, most commonly, rearrangement and cyclization of prenyl diphosphate substrates, forming linear and cyclic hydrocarbons. Moreover, a single TPS often produces several minor products in addition to a dominant product. We characterized the catalytic profiles of Hyoscyamus muticus premnaspirodiene synthase (HPS) and compared it with the profile of a closely related TPS, Nicotiana tabacum 5-epi-aristolochene synthase (TEAS). The profiles of two previously studied HPS and TEAS mutants, each containing nine interconverting mutations, dubbed HPS-M9 and TEAS-M9, were also characterized. All four TPSs were compared under varying temperature and pH conditions. In addition, we solved the X-ray crystal structures of TEAS and a TEAS quadruple mutant complexed with substrate and products to gain insight into the enzymatic features modulating product formation. These informative structures, along with product profiles, provide new insight into plant TPS catalytic promiscuity.
机译：植物萜烯合酶（TPS）家族负责各种具有多种生物学功能的萜类天然产物的生物合成。 TPS催化异戊二烯基二磷酸底物的电离，最常见的是重排和环化，形成直链和环状烃。此外，单个TPS除了占主导地位的产品外，通常还会生产多个次要产品。我们表征了Hyscyamus muticus premnaspirodiene合酶（HPS）的催化图谱，并将其与密切相关的TPS烟草5-烟草-aristolochene合酶（TEAS）的图谱进行了比较。还描述了两个先前研究的HPS和TEAS突变体的概况，每个突变体包含9个相互转换的突变，称为HPS-M9和TEAS-M9。在不同的温度和pH条件下比较了所有四种TPS。此外，我们解决了TEAS的X射线晶体结构以及与底物和产物复合的TEAS四重突变体，以深入了解调节产物形成的酶学特征。这些信息结构以及产品概况可为植物TPS催化混杂提供新的见解。

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