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首页> 外文期刊>The Journal of Antibiotics: An International Journal >Prevalence of mutations conferring resistance among multi- and extensively drug-resistant Mycobacterium tuberculosis isolates in China
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Prevalence of mutations conferring resistance among multi- and extensively drug-resistant Mycobacterium tuberculosis isolates in China

机译：在中国，多重和广泛耐药结核分枝杆菌菌株中赋予耐药性的突变普遍存在

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摘要

To identify the mutations in multi- and extensively drug-resistant tuberculosis isolates and to evaluate the use of molecular markers of resistance, we analyzed 257 multi- and extensively drug-resistant isolates and 64 pan-sensitive isolates from 23 provinces in China. Seven loci associated with drug resistance, including rpoB for rifampin (RIF), katG, inhA and oxyR-ahpC for isoniazid (INH), gyrA and gyrB for ofloxacin (OFX), and rrs for kanmycin (KAN), were examined by DNA sequencing. Compared with the phenotypic data, the sensitivity and specificity for DNA sequencing were 91.1% and 98.4% for RIF, 80.2% and 98.4% for INH, 72.2% and 98.3% for OFX and 40% and 98.2% for KAN, respectively. The most common mutations found in RIF, INH, OFX and KAN resistance were Ser531Leu (48.2%) in rpoB, Ser315Thr (49.8%) in katG, C(-15)T (10.5%) in inhA, Asp94Gly (20.3%), Asp94Ala (12.7%) and Ala90Val (21.5%) in gyrA, and A1401G (40%) in rrs. This molecular information will be helpful to establish new molecular biology-based methods for diagnosing multi- and extensively drug-resistant tuberculosis in China.
机译：为了鉴定耐多药和广泛耐药结核病菌株的突变并评估耐药性分子标记的使用，我们分析了中国23个省的257种耐多药和广泛耐药菌株和64种泛敏感菌株。通过DNA测序检查了七个与耐药相关的基因座，包括利福平（RIF）的rpoB，异烟肼（INH）的katG，inhA和oxyR-ahpC，氧氟沙星（OFX）的gyrA和gyrB以及甘霉素（KAN）的rrs。 。与表型数据相比，RIF的DNA测序灵敏度和特异性分别为91.1％和98.4％，INH的灵敏度和特异性分别为80.2％和98.4％，OFX的灵敏度为72.2％和98.3％，KAN的灵敏度为90％和98.2％。在RIF，INH，OFX和KAN抗性中发现的最常见突变是rpoB中的Ser531Leu（48.2％），katG中的Ser315Thr（49.8％），inhA中的C（-15）T（10.5％），Asp94Gly（20.3％）， gyrA中的Asp94Ala（12.7％）和Ala90Val（21.5％），以及rrs中的A1401G（40％）。这些分子信息将有助于建立基于分子生物学的新方法，以诊断中国的多重耐药和广泛耐药性结核病。

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