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首页> 外文期刊>The Journal of Antibiotics: An International Journal >Bioconversion of AS1387392: bioconversion studies involving Amycolatopsis azurea JCM 3275.
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Bioconversion of AS1387392: bioconversion studies involving Amycolatopsis azurea JCM 3275.

机译：AS1387392的生物转化：涉及Amycolatopsis azurea JCM 3275的生物转化研究。

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摘要

We screened actinomycetes capable of converting AS1387392 to AS1429716 and identified those strains capable of hydroxylation. Amycolatopsis azurea JCM 3275 was found to be a particularly efficient strain, capable of converting AS1387392 to AS1429716, with a yield of 44% after 9 h. This strain can metabolize not only the hydroxylation of phenylalanine at the meta and para positions but also the reduction of hydroxyketones, as shown by the isolation of bioconversion products. Examination of more suitable conversion conditions showed that pH 7.8 and 25 degrees C were the optimum pH and temperature for bioconversion, respectively. We also demonstrated the effect of carbon and nitrogen sources in the culture media on hydroxylation. Using this strain, we were able to efficiently produce AS1429716 as a chemical template. Further derivatization studies may provide more effective, safer immunosuppressants than those that are currently on-market.
机译：我们筛选了能够将AS1387392转换为AS1429716的放线菌，并鉴定了能够羟基化的菌株。发现Amycolatopsis azurea JCM 3275是一种特别有效的菌株，能够将AS1387392转化为AS1429716，9小时后产率为44％。如分离的生物转化产物所示，该菌株不仅可以代谢间位和对位的苯丙氨酸的羟基化，而且还可以代谢羟基酮的还原。检查更合适的转化条件表明，pH 7.8和25摄氏度分别是生物转化的最佳pH和温度。我们还证明了培养基中碳源和氮源对羟基化的影响。使用此菌株，我们能够有效地生产AS1429716作为化学模板。进一步的衍生化研究可能会提供比目前市场上更有效，更安全的免疫抑制剂。

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