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首页> 外文期刊>The Journal of Antibiotics: An International Journal >Pilot-plant cultivation of Streptomyces griseus producing homologues of nonactin by precursor-directed biosynthesis and their identification by LC/MS-ESI
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Pilot-plant cultivation of Streptomyces griseus producing homologues of nonactin by precursor-directed biosynthesis and their identification by LC/MS-ESI

机译：通过前体定向生物合成生产链霉菌非肌动蛋白同源物的灰链霉菌的中试栽培及其LC / MS-ESI鉴定

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摘要

Precursor-directed biogenetic approach was used to produce a range of nonactin homologues in a 50 l fermentor cultivation of strain Streptomyces griseus 34/249 obtained by UV mutagenesis. The production medium contained sodium propionate, isobutyrate and isovalerate as individual precursors, and 10 g l-1 Diaion HP20 styrene-divinylbenzene resin that maintains suitable precursor concentration by reversibly adsorbing and releasing it. The produced nonactin homologues were separated on two C18 reversed-phase liquid chromatography columns in series and analyzed by MS with ESI source in the positive ion mode. Formation of doubly charged ions was suppressed by an excess of Na+ ions throughout the process. The production of the homologues increased up to day 5 and then it leveled off. Cultivations with individual precursors yielded a total of 18 nonactin homologues whose spectrum depended on the precursor used. The total production of the homologues was lowered but their spectrum was shifted to higher-molecular-weight compounds.
机译：在通过紫外线诱变获得的50升灰霉菌链霉菌34/249菌株的50升发酵罐培养物中，使用前体定向的生物遗传学方法产生了一系列非肌动蛋白同源物。生产介质包含分别作为单独的前体的丙酸钠，异丁酸钠和异戊酸钠，以及10 g l-1 Diaion HP20苯乙烯-二乙烯基苯树脂，该树脂通过可逆地吸附和释放来保持合适的前体浓度。产生的非肌动蛋白同源物在两个串联的C18反相液相色谱柱上分离，并通过MS和ESI源在正离子模式下进行分析。在整个过程中，过量的Na +离子抑制了双电荷离子的形成。直到第5天，同源物的产量增加，然后趋于稳定。用单独的前体进行培养产生了总共18种非肌动蛋白同源物，其光谱取决于所用的前体。同源物的总产量降低了，但是它们的光谱却转移到了更高分子量的化合物上。

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