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首页> 外文期刊>The Journal of Antibiotics: An International Journal >New milbemycins from mutant Streptomyces bingchenggensis X-4.
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New milbemycins from mutant Streptomyces bingchenggensis X-4.

机译：突变的宾城链霉菌X-4的新米尔倍霉素。

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The fermentation broth (301) was filtered. The resulting cake was washed with water, and both filtrate and wash were discarded. Methanol (101) was used to extract the washed cake. The MeOH extract was evaporated under reduced pressure to - 21 at 45 °C and the resulting concentrate was extracted three times using an equal volume of EtOAc. The combined EtOAc phase was concentrated under reduced pressure to yield 30 g of oily substances. The residual oily substance was chromatographed on silica gel (Qingdao Haiyang Chemical Group, Qingdao, China; 100-200 mesh) and eluted with a petroleum ether-acetone mixture (100:0-50:50, v/v). The fractions eluted with the petroleum ether-acetone mixture (90:10, v/v) were combined and evaporated to obtain fraction I and the fractions eluted with the petroleum ether-acetone mixture (85:15, v/v) were pooled and concentrated to give fraction II.
机译：过滤发酵液（301）。用水洗涤所得的饼，并丢弃滤液和洗液。使用甲醇（101）提取洗涤的饼。将MeOH萃取物在45℃下减压蒸发至-21，然后将所得浓缩物用等体积的EtOAc萃取3次。将合并的EtOAc相减压浓缩，得到30g油状物质。将残余的油状物在硅胶上色谱分离（青岛海阳化学集团，青岛; 100-200目），并用石油醚-丙酮混合物（100：0-50：50，v / v）洗脱。合并用石油醚-丙酮混合物（90：10，v / v）洗脱的级分并蒸发以获得级分I，并且将用石油醚-丙酮混合物（85：15，v / v）洗脱的级分合并并合并。浓缩得到级分II。

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