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首页> 外文期刊>The Journal of Antibiotics: An International Journal >Substrate specificity of benzamide synthetase involved in 4-hydroxy-3-nitrosobenzamide biosynthesis.
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Substrate specificity of benzamide synthetase involved in 4-hydroxy-3-nitrosobenzamide biosynthesis.

机译：参与4-羟基-3-亚硝基苯甲酰胺生物合成的苯甲酰胺合成酶的底物特异性。

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摘要

Glutamine-dependent amidotransferase (Gn-AT) catalyzes the transfer of the amido nitrogen of glutamine to an acceptor substrate to produce glutamate and an aminated product. Although most Gn-ATs are involved in biosyntheses of primary metabolites, some are important in the synthesis of secondary metabolites. Recently, a Gn-AT (NspN) was discovered in the biosynthetic pathway of 4-hydroxy-3-nitorosobenzamide in Streptomyces murayamaensis. NspN converts 3-amino-4-hydroxybenzoic acid (3,4-AHBA) to 3-amino-4-hydroxybenzamide. Here, we report the amino-acceptor substrate specificity of NspN. NspN could use several benzoic acid derivatives as amino-acceptor substrates to produce corresponding benzamides and catalyze the amidation of several carboxylate-type phenylpropanoids, such as p-coumaric acid, cinnamic acid and caffeic acid. NspN showed the highest activity toward its natural substrate 3,4-AHBA among the substrates examined. NspN and related bacterial Gn-ATs may be useful in developing combinatorial biosynthetic strategies for benzamide derivatives, which could, in turn, be used as therapeutic agents for a variety of diseases.
机译：谷氨酰胺依赖性酰胺基转移酶（Gn-AT）催化谷氨酰胺的酰胺基氮转移到受体底物上，从而产生谷氨酸盐和胺化产物。尽管大多数Gn-AT参与一级代谢产物的生物合成，但有些在二级代谢产物的合成中很重要。近来，在村山链霉菌的4-羟基-3-氮杂苯磺酰胺的生物合成途径中发现了Gn-AT（NspN）。 NspN将3-氨基-4-羟基苯甲酸（3,4-AHBA）转化为3-氨基-4-羟基苯甲酰胺。在这里，我们报告NspN的氨基受体底物特异性。 NspN可以使用几种苯甲酸衍生物作为氨基受体底物，以产生相应的苯甲酰胺，并催化几种羧酸盐型苯基丙烷（如对香豆酸，肉桂酸和咖啡酸）的酰胺化反应。在检查的底物中，NspN对其天然底物3,4-AHBA表现出最高的活性。 NspN和相关的细菌Gn-ATs可能用于开发苯甲酰胺衍生物的组合生物合成策略，而后者又可以用作多种疾病的治疗剂。

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