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首页> 外文期刊>The Journal of Antibiotics: An International Journal >Microbispora thailandensis sp. nov., an actinomycete isolated from cave soil
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Microbispora thailandensis sp. nov., an actinomycete isolated from cave soil

机译：小双孢菌thailandensis sp。十一月，从洞穴土壤中分离出来的放线菌

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摘要

The taxonomic position of actinomycete strain NN276 T, isolated from cave soil, was studied using the polyphasic taxonomic approach. A phylogenetic tree based on 16S ribosomal RNA (rRNA) gene sequences showed that the isolate formed a distinct evolutionary linage with the genus Microbispora, with M. mesophila JCM 3151 T as its closest phylogenetic neighbor (97.9% similarity). The organism contained meso-diaminopimelic acid and the N-acetyl type of peptidoglycan. Madurose was detected in the whole-cell hydrolasate. The predominant menaquinones were MK-9(H 4), MK-9(H 2) and MK-9. Mycolic acids were not detected. Major phospholipids were diphosphatidylglycerol, hydroxy- phosphatidylethanolamine, phosphatidylethanolamine, phosphatidylinositol and phosphatidylinositol mannoside. The major cellular fatty acid was iso-C 16: 0 and GC content 70 mol%. DNA-DNA hybridization demonstrated that the isolate was distinct from M. mesophila JCM 3151 T. On the basis of phenotypic and genotypic data, it is proposed that strain NN276 T represents a novel species of the genus Microbispora, hence the name Microbispora thailandensis sp. nov. The type strain is strain NN276 T (BCC 41490 T NRRL B-24806 T NBRC 107569 T).
机译：使用多相分类方法研究了从洞穴土壤中分离的放线菌菌株NN276 T的分类位置。基于16S核糖体RNA（rRNA）基因序列的系统进化树显示，该分离物与Microbispora属形成了独特的进化谱系，其中嗜麦芽孢杆菌JCM 3151 T为最接近的系统进化邻居（相似性为97.9％）。该生物体包含内消旋二氨基庚二酸和N-乙酰基肽聚糖。在全细胞水解酶中检测到了马杜糖。主要的甲萘醌为MK-9（H 4），MK-9（H 2）和MK-9。未检测到霉菌酸。主要的磷脂是二磷脂酰甘油，羟基磷脂酰乙醇胺，磷脂酰乙醇胺，磷脂酰肌醇和磷脂酰肌醇甘露糖苷。主要的细胞脂肪酸为iso-C 16：0，GC含量为70 mol％。 DNA-DNA杂交表明该分离物不同于嗜麦芽孢杆菌JCM 3151T。根据表型和基因型数据，提出菌株NN276 T代表微双孢菌属的一种新种，因此命名为Microbispora thailandensis sp。十一月类型菌株是菌株NN276 T（BCC 41490 T NRRL B-24806 T NBRC 107569 T）。

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