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首页> 外文期刊>The Journal of Antibiotics: An International Journal >MS/MS-based networking and peptidogenomics guided genome mining revealed the stenothricin gene cluster in Streptomyces roseosporus
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MS/MS-based networking and peptidogenomics guided genome mining revealed the stenothricin gene cluster in Streptomyces roseosporus

机译：基于MS / MS的网络和肽基因组学指导的基因组挖掘揭示了玫瑰链霉菌中的stenothricin基因簇

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摘要

Most (75%) of the anti-infectives that save countless lives and enormously improve quality of life originate from microbes found in nature. Herein, we described a global visualization of the detectable molecules produced from a single microorganism, which we define as the 'molecular network' of that organism, followed by studies to characterize the cellular effects of antibacterial molecules. We demonstrate that Streptomyces roseosporus produces at least four non-ribosomal peptide synthetase-derived molecular families and their gene subnetworks (daptomycin, arylomycin, napsamycin and stenothricin) were identified with different modes of action. A number of previously unreported analogs involving truncation, glycosylation, hydrolysis and biosynthetic intermediates and/or shunt products were also captured and visualized by creation of a map through MS/MS networking. The diversity of antibacterial compounds produced by S. roseosporus highlights the importance of developing new approaches to characterize the molecular capacity of an organism in a more global manner. This allows one to more deeply interrogate the biosynthetic capacities of microorganisms with the goal to streamline the discovery pipeline for biotechnological applications in agriculture and medicine. This is a contribution to a special issue to honor Chris Walsh's amazing career.
机译：挽救无数生命并极大改善生活质量的大多数抗感染药（75％）来自自然界中发现的微生物。在这里，我们描述了由单个微生物产生的可检测分子的全局可视化，我们将其定义为该生物的“分子网络”，然后进行研究以表征抗菌分子的细胞作用。我们证明玫瑰链霉菌产生至少四个非核糖体肽合成酶衍生的分子家族和他们的基因子网络（达托霉素，arylomycin，napsamycin和stenothricin）被确定具有不同的作用方式。通过MS / MS网络创建图谱，还捕获了许多以前未报道的类似物，包括截短，糖基化，水解和生物合成中间体和/或分流产物。玫瑰孢链球菌产生的抗菌化合物的多样性凸显了开发新方法以更全面的方式表征生物分子能力的重要性。这使得人们可以更深入地研究微生物的生物合成能力，以期简化用于农业和医学生物技术应用的发现流程。这是对纪念克里斯·沃尔什（Chris Walsh）惊人职业生涯的特刊的贡献。

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