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首页> 外文期刊>The Journal of Antibiotics: An International Journal >A 7.6 kb DNA region from Streptomyces kasugaensis M338-M1 includes some genes responsible kasugamycin biosynthesis
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A 7.6 kb DNA region from Streptomyces kasugaensis M338-M1 includes some genes responsible kasugamycin biosynthesis

机译：春日链霉菌M338-M1的一个7.6 kb DNA区域包含一些与春节霉素生物合成有关的基因

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摘要

A 7.6 kb PstI-KpnI DNA fragment including a sequence highly similar to kasugamycin acetyltransferase gene (kac) was isolated from Streptomyces kasugaensis M338-M1 and sequenced. Nine open reading frames (ORFs), designated as ORF A, B, C, D, E, F, G, H and I, were recognized in this region, although ORF A was incomplete. ORF G runs in the opposite direction to the others. The amino acid sequence deduced from ORF H showed 98% similarity to that of the kasugamycin acetyltransferase from S. kasugaensis MB273-C4, another kasugamycin (KSM) producer. Transformation of E. coli JM109 with ORF H made the strain highly resistant to KSM. The deduced amino acid sequences of the ORF A, C and D products were similar, respectively, to glucosyltransferase I from E. coli (26%), beta-alanine:pyruvate transaminase from Pseudomonas putida (32%) and dTDP-D-glucose 4,6-dehydratase (StrE) from Streptomyces griseus (37%). The strE-like ORF (ORF D) seems to be the gene responsible for formation of the 6-deoxy structure of the kasugamine moiety. ORF A and ORF C are also likely to have roles in KSM biosynthesis. Taken together, our analyses strongly suggest that this DNA region includes at least a part of the gene cluster of KSM biosynthesis. [References: 29]
机译：从春日链霉菌M338-M1中分离出一个7.6 kb的PstI-KpnI DNA片段，该片段与春日霉素乙酰转移酶基因（kac）高度相似。尽管ORFA不完整，但在该区域中识别出九个开放阅读框（ORF），分别命名为ORFA，B，C，D，E，F，G，H和I。 ORF G的运行方向与其他方向相反。从ORF H推导的氨基酸序列显示出与另一株春日霉素（KSM）生产者春日链霉菌MB273-C4的春日霉素乙酰基转移酶的98％相似性。用ORF H转化大肠杆菌JM109使该菌株对KSM具有高度抗性。 ORF A，C和D产物的推导氨基酸序列分别类似于大肠杆菌的葡糖基转移酶I（26％），恶臭假单胞菌的β-丙氨酸：丙酮酸转氨酶（32％）和dTDP-D-葡萄糖灰链霉菌（Streptomyces griseus）的4，6-脱水酶（StrE）（37％）。类似strE的ORF（ORF D）似乎是负责形成春日明部分6-脱氧结构的基因。 ORF A和ORF C也可能在KSM生物合成中起作用。两者合计，我们的分析强烈表明，该DNA区域至少包含KSM生物合成基因簇的一部分。 [参考：29]

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