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首页> 外文期刊>The Journal of craniofacial surgery >Comparison between genetic portraits of osteoblasts derived from primary cultures and osteoblasts obtained from human pulpar stem cells.
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Comparison between genetic portraits of osteoblasts derived from primary cultures and osteoblasts obtained from human pulpar stem cells.

机译：从原代培养获得的成骨细胞与从人类牙髓干细胞获得的成骨细胞的遗传肖像之间的比较。

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摘要

Harvesting bone for autologous grafting is a daily problem encountered by craniofacial and oral surgeons. Stem cells derived from human dental pulp are able to differentiate in osteoblasts and are a potential source of autologous bone produced in vitro. However, as stem cells are characterized by self-renewing and commitment in several cellular subtypes (ie, pluripotential differentiation), some concerns may arise as regards their potential uncontrolled proliferation. To screen the behavior of osteoblasts derived from human pulpar stem cells (ODHPSCs), we used microarray techniques to identify genes that are differently regulated in ODHPSC in comparison to normal osteoblasts (NOs). Osteoblasts derived from human pulpar stem cells were obtained from human dental pulp, and cells were selected using a cytometer. The cell profile was c-kit+/CD34+/STRO-1+/CD45-. These cells were capable of differentiation of osteoblasts in vitro. By using DNA microarrays containing 19,200 genes, we identified in ODHPSC somegenes whose expression was significantly up- and downregulated compared to NO. The differentially expressed genes have different functional activities: (a) cell differentiation, (b) developmental maturation, (c) cell adhesion, and (d) production of cytoskeleton elements. Thus, some molecular differences exist between NO and ODHPSC, although the previously considered histologic parameters show a normal phenotype.
机译：颅面外科和口腔外科医师每天都面临着为自体移植而采集骨头的难题。源自人类牙髓的干细胞能够在成骨细胞中分化，并且是体外产生的自体骨的潜在来源。然而，由于干细胞的特征在于几种细胞亚型的自我更新和承诺（即多能分化），因此关于其潜在的不受控制的增殖可能引起一些担忧。为了筛选源自人类牙髓干细胞（ODHPSCs）的成骨细胞的行为，我们使用微阵列技术来鉴定与正常成骨细胞（NOs）相比在ODHPSC中调控不同的基因。从人牙髓获得源自人牙髓干细胞的成骨细胞，并使用细胞仪选择细胞。细胞概况是c-kit + / CD34 + / STRO-1 + / CD45-。这些细胞能够在体外分化成骨细胞。通过使用包含19,200个基因的DNA芯片，我们在ODHPSC中鉴定了一些基因的表达与NO相比显着上调和下调。差异表达的基因具有不同的功能活性：（a）细胞分化，（b）发育成熟，（c）细胞粘附和（d）细胞骨架元素的产生。因此，尽管先前认为的组织学参数显示正常表型，但NO和ODHPSC之间存在一些分子差异。

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