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首页> 外文期刊>The Journal of heart valve disease >Tissue engineering of heart valves: biomechanical and morphological properties of decellularized heart valves.
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Tissue engineering of heart valves: biomechanical and morphological properties of decellularized heart valves.

机译：心脏瓣膜的组织工程：脱细胞心脏瓣膜的生物力学和形态学特性。

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BACKGROUND AND AIM OF THE STUDY: Biological scaffolds are widely used in the process of cardiac valve tissue engineering. Scaffold characteristics are decisive for valve durability. Herein, the influence of three different decellularization protocols on the morphological and biomechanical properties of porcine pulmonary valve conduits was evaluated. METHODS: Pulmonary valve conduits were decellularized with 1% sodium deoxycholate (SD), 1% sodium dodecylsulfate (SDS), or 0.05% trypsin/0.02% EDTA. The degree of decellularization and morphological integrity of the treated pulmonary valve cusp, wall and myocardial cuff were analyzed with hematoxylin and eosin staining, Movat-Pentachrome staining, electron microscopy, and DNA assay. The conservation of extracellular matrix (ECM) proteins was evaluated by immunohistochemical staining against collagens I and IV, and laminin. The biomechanical properties of the obtained scaffolds were evaluated using uniaxial tension tests. Native grafts served as controls. RESULTS: All treatments resulted in complete decellularization of the cusp, whereas only SD and SDS treatments were able to remove completely all cells from the pulmonary valve wall and subvalvular myocardial cuff. The morphological integrity and preservation of ECM proteins was clearly superior in both detergent-treated groups. Enzyme treatment resulted in destruction of the basement membrane. Wall longitudinal tension parameters (stiffness, elasticity modulus, ultimate force; stress and strain) were significantly inferior in the trypsin/EDTA group (p < 0.05). No significant differences were observed between detergent-treated and native samples. The results of transversal tension parameters were comparable in all groups. CONCLUSION: Both, SD and SDS treatment of the pulmonary valve may better preserve the morphological and biomechanical properties of the scaffold than the chosen enzymatic treatment. In the authors' opinion, detergent-based decellularization should be used in preference to enzyme treatment inthe tissue engineering of heart valves.
机译：研究背景和目的：生物支架广泛用于心脏瓣膜组织工程的过程。脚手架的特性对于阀门的耐用性至关重要。本文中，评估了三种不同的脱细胞方案对猪肺动脉瓣膜导管的形态和生物力学性能的影响。方法：肺动脉瓣导管用1％脱氧胆酸钠（SD），1％十二烷基硫酸钠（SDS）或0.05％胰蛋白酶/0.02% EDTA脱细胞。用苏木精和曙红染色，Movat-Pentachrome染色，电子显微镜和DNA分析法分析治疗的肺动脉瓣尖，壁和心肌袖带的脱细胞度和形态完整性。通过针对胶原蛋白I和IV和层粘连蛋白的免疫组织化学染色评估了细胞外基质（ECM）蛋白的保守性。使用单轴拉伸试验评估获得的支架的生物力学性能。天然移植物用作对照。结果：所有处理均导致尖瓣完全脱细胞，而只有SD和SDS处理能够从肺动脉瓣壁和瓣膜下袖带完全清除所有细胞。在两个去污剂处理组中，ECM蛋白的形态完整性和保存性均明显优于其他洗涤剂组。酶处理导致基底膜的破坏。胰蛋白酶/ EDTA组的壁纵向张力参数（刚度，弹性模量，极限力，应力和应变）明显较差（p <0.05）。经去污剂处理的样品与天然样品之间未观察到显着差异。横向张力参数的结果在所有组中都是可比的。结论：SD和SDS治疗肺动脉瓣可能比选择的酶处理方法更好地保留支架的形态和生物力学特性。作者认为，在心脏瓣膜的组织工程中，应优先使用基于去污剂的脱细胞，而不是酶处理。

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