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首页> 外文期刊>The Journal of dermatology >Detection of mycobacterial DNA in cervical granulomatous lymphadenopathy from formalin-fixed, paraffin-embedded tissue by PCR.
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Detection of mycobacterial DNA in cervical granulomatous lymphadenopathy from formalin-fixed, paraffin-embedded tissue by PCR.

机译：通过PCR检测福尔马林固定石蜡包埋的组织中子宫颈肉芽肿性淋巴结病的分枝杆菌DNA。

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摘要

Cervical tuberculous lymphadenitis is the most common form of inflammatory neck mass in Korea. The diagnosis of tuberculosis requires proof of the presence of Mycobacterium tuberculosis by acid-fast staining or bacterial growth in culture. However, these are often difficult in cervical tuberculous lymphadenitis. The aim of this study was to investigate the value of the polymerase chain reaction (PCR) technique for detection of mycobacteria in routinely processed tissue sections of cervical granulomatous lymphadenopathy. In this retrospective study, twenty formalin-fixed, paraffin-embedded biopsy specimens from clinically and/or histopathologically diagnosed cervical granulomatous lymphadenopathy were analyzed for mycobacterial DNA by PCR. Two different primers to amplify mycobacterial-common 383-base pair (bp) DNA and Mycobacterium tuberculosis-complex-specific 123-bp DNA were used. Positive PCR products were sequenced directly. Mycobacterial-common DNA (383-bp positive) was found in 10 of the 20 cases. Among them, 7 cases were PCR positive with both primer sets. These seven cases can be considered as tuberculosis. The other three cases indicated possible atypical mycobacteriosis. PCR is a useful technique for the demonstration of mycobacterial DNA fragments in patients with clinically suspected cervical tuberculous lymphadenitis who have acid fast-negative histology and/or unsuccessful mycobacterial cultures.
机译：颈椎淋巴结炎是韩国炎性颈部肿块最常见的形式。结核病的诊断需要通过耐酸染色或培养物中细菌的生长来证明结核分枝杆菌的存在。然而，这些在宫颈结核性淋巴结炎中通常是困难的。这项研究的目的是调查聚合酶链反应（PCR）技术在宫颈肉芽肿性淋巴结病的常规处理组织切片中检测分枝杆菌的价值。在这项回顾性研究中，通过PCR分析了20份临床和/或组织病理学诊断为宫颈肉芽肿性淋巴结病的福尔马林固定，石蜡包埋的活检标本的分枝杆菌DNA。使用了两种不同的引物来扩增分枝杆菌常见的383个碱基对（bp）DNA和结核分枝杆菌复合物特异的123-bp DNA。阳性PCR产物直接测序。在20例病例中有10例发现了分枝杆菌共有的DNA（383 bp阳性）。其中，有两个引物组均为PCR阳性的7例。这七个病例可以认为是结核病。其他三例表明可能是非典型的分枝杆菌病。 PCR是一种有用的技术，可用于在临床疑似宫颈结核性淋巴结炎，酸快速阴性组织学和/或分枝杆菌培养失败的患者中证明分枝杆菌DNA片段。

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