• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>The Journal of Horticultural Science & Biotechnology >Expression of anti-oxidant enzyme genes in scald-resistant 'Belfort' and scald-susceptible 'Granny Smith' apples during cold storage.
【24h】

Expression of anti-oxidant enzyme genes in scald-resistant 'Belfort' and scald-susceptible 'Granny Smith' apples during cold storage.

机译：冷藏期间，耐烫伤的'Belfort'和易受烫伤的'Granny Smith'苹果中抗氧化酶基因的表达。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Real-Time PCR was used to determine whether the levels of mRNAs coding for anti-oxidant enzymes varied in apple peel during post-harvest cold storage, and if these were correlated with the occurrence of apple scald symptoms. Levels of the scald-related compounds, alpha -farnesene and conjugated trienes (CT; particularly, CT281 and CT258), were determined in fruit peel of the scald-resistant apple 'Belfort' and the scald-susceptible apple 'Granny Smith' during storage. alpha -Farnesene levels varied, irrespective of the incidence of scald, whereas the levels of CTs were correlated with the degree of scald. On the other hand, the level of H2O2 in 'Granny Smith' apple peel increased gradually, but significantly, from harvest during storage, whereas this value did not vary in 'Belfort' peel. In both apple cultivars, the levels of mRNA for Mn superoxide dismutase (MnSOD) decreased with storage time, whereas the levels of ascorbate peroxidase (APX) mRNA did not vary. In 'Granny Smith' apples, mRNA levels for catalase (CAT) increased dramatically (7.8-fold) late in the storage period. Monodehydro-ascorbate reductase (MDHAR) and glutathione reductase (GR) mRNA levels also increased greatly (12- and 18.8-fold, respectively) early in storage. Conversely, in 'Belfort' apples the transcript levels of these genes did not change during storage. These results indicate that scaldresistance in 'Belfort' apples was not directly related to higher levels of mRNAs for anti-oxidant enzymes. However, the higher levels of H2O2 in 'Granny Smith'apples was positively correlated with the higher levels of mRNAs for the anti-oxidant enzymes CAT, GR and MDHAR, suggesting direct regulation by the level of H2O2 on transcription of these anti-oxidant enzyme genes.
机译：实时PCR用于确定收获后冷藏期间苹果皮中编码抗氧化酶的mRNA的水平是否变化，以及是否与苹果烫伤症状的发生有关。在存储过程中，测定了耐烫伤的苹果“ Belfort”和易烫伤的苹果“ Granny Smith”的果皮中与烫伤相关的化合物α-法呢烯和共轭三烯（CT;尤其是CT281和CT258）的水平。 。不论烫伤的发生率如何，α-法呢烯水平都不同，而CT的水平与烫伤程度相关。另一方面，“格兰尼·史密斯”苹果皮中的H2O2水平逐渐增加，但从储存期间的收成来看是明显增加的，而“百福”果皮中的该值没有变化。在两个苹果品种中，锰超氧化物歧化酶（MnSOD）的mRNA水平均随储存时间的延长而下降，而抗坏血酸过氧化物酶（APX）的mRNA水平则没有变化。在“格兰尼·史密斯”（Granny Smith）苹果中，过氧化氢酶（CAT）的mRNA水平在储存期后期急剧增加（7.8倍）。储存初期，单脱氢抗坏血酸还原酶（MDHAR）和谷胱甘肽还原酶（GR）mRNA水平也大大提高（分别为12倍和18.8倍）。相反，在“百福”苹果中，这些基因的转录水平在储存过程中没有变化。这些结果表明，“百福”苹果的抗烫伤性与抗氧化酶的高水平mRNA没有直接关系。然而，“格兰尼·史密斯”苹果中较高的H2O2与抗氧化剂CAT，GR和MDHAR的较高的mRNA水平呈正相关，这表明H2O2的水平直接调节了这些抗氧化剂的转录基因。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号