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首页> 外文期刊>The Journal of Horticultural Science & Biotechnology >Diversity of S-RNase genes and S-haplotypes in Japanese plum (Prunus salicina Lindl.)
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Diversity of S-RNase genes and S-haplotypes in Japanese plum (Prunus salicina Lindl.)

机译：李子中S-RNase基因和S-单倍型的多样性

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摘要

This report demonstrates the diversity of S-haplotypes in Japanese plum (P. salicina) by molecular cloning of genomic DNAs and cDNAs that encode S-RNases. Nine different DNA fragments, designated as Sa-Si, were obtained from 17 Japanese plum cultivars by PCR with an S-RNase gene-specific primer set, Pru-C2 and PCE-R. Eleven different S-haplotypes were found in these cultivars. The banding patterns obtained with another S-RNase gene-specific primer set, Pru-T2 and PCE-R, corresponded to the S-haplotypes predicted from the Pru-C2 and PCE-R primer set. Several cultivars had the same S-haplotypes. Partial genomic DNAs for eight S-RNase genes and cDNAs for two S-RNases were cloned and sequenced. Deduced amino acid sequences contained conserved regionsamong the rosaceous S-RNases. Comparisons of the sequences from cDNAs and genomic DNAs revealed the presence of two introns in the S-RNase genes of Japanese plum as in other Prunus S-RNase genes. Pollination incompatibility groups and self-compatibilityin Japanese plum were discussed with reference to the S-haplotypes.
机译：该报告通过分子克隆编码S-RNase的基因组DNA和cDNA证明了日本李（P. salicina）中S-单倍型的多样性。使用S-RNase基因特异性引物对Pru-C2和PCE-R，通过PCR从17个日本李子品种中获得了9个不同的DNA片段，称为Sa-Si。在这些品种中发现了十一种不同的S-单倍型。用另一种S-RNase基因特异性引物组Pru-T2和PCE-R获得的条带模式对应于从Pru-C2和PCE-R引物组预测的S-单倍型。几个品种具有相同的S单倍型。克隆并测序了八个S-RNase基因的部分基因组DNA和两个S-RNase的cDNA。推导的氨基酸序列包含玫瑰红质S-RNase中的保守区域。来自cDNA和基因组DNA的序列比较表明，与其他李属S-RNase基因一样，日本李子的S-RNase基因中存在两个内含子。参照S-单倍型讨论了授粉不相容群体和日本李子的自相容性。

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