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首页> 外文期刊>The Journal of Horticultural Science & Biotechnology >Phylogenetic relationships among cultivars of Prunus persica based on internal transcribed spacer (ITS) sequences of nuclear ribosomal DNA.
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Phylogenetic relationships among cultivars of Prunus persica based on internal transcribed spacer (ITS) sequences of nuclear ribosomal DNA.

机译：基于核糖体DNA的内部转录间隔区（ITS）序列的桃（Perius persica）品种之间的亲缘关系。

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摘要

The nucleotide sequences of the internal transcribed spacer (ITS) regions of nuclear rDNA were determined for 51 peach cultivars (Prunus persica L. Batch) from different eco-geographical regions of China and other countries, and compared with three related species (P. ferganensis, P. davidiana, and P. mume) and one natural inter-specific hybrid ('Baishanbitao'). The ITS 1 region in 51 peach cultivars was 229-233 bp in length, the 5.8S rDNA gene was 85 bp, and the ITS 2 region was 278-281 bp. There were clear differences in the length of the ITS 1 region between peach cultivars originating from different eco-geographical regions. All North American and Japanese cultivars had ITS 1 regions of equal length at 229 bp and 230 bp, respectively, while Chinese cultivars showed various lengths of the ITS 1 region, ranging from 229-233 bp. Three groups were defined, based on phylogenetic analysis. Group I contained all the yellow peaches, except one local inter-specific hybrid Chinese yellow peach ('Baishanbitao'), plus some Chinese ornamental and white peaches. Group II was divided into two sub-Groups. Sub-Group 1 contained only two local northwestern Chinese cultivars, and sub-Group 2 included mainly flat peaches and local northern Chinese cultivars. Also, two related species, P. davidiana and P. ferganensis, and a dwarf peach (P. persica var. densa) were clustered into sub-Group 2. Group III contained mainly Japanese white peach cultivars. P. mume was unique and not assigned to any Group. It is suggested that peaches with the genetic background of P. davidiana and P. ferganensis could be a variety of P. persica. 'Zhanghuang 7', 'Wuyuexian', and 'Baimangpantao' may be the oldest cultivars among those tested in this study, and 'Baishanbitao' is a P. davidiana x P. persica hybrid.
机译：确定了中国和其他国家/地区不同生态地理区域的51个桃品种（Prunus persica L. Batch）核rDNA的内部转录间隔区（ITS）的核苷酸序列，并与三个相关物种（ P。ferganensis ， da.P。davidiana 和 P。mume ）和一个天然的种间杂种（'Baishanbitao'） 。 51个桃子品种的ITS 1区域长度为229-233 bp，5.8S rDNA基因为85 bp，ITS 2区域为278-281 bp。来自不同生态地理区域的桃子品种在ITS 1区域的长度上存在明显差异。所有北美和日本品种的ITS 1区域的长度分别相等，分别为229 bp和230 bp，而中国品种的ITS 1区域的长度则不同，范围为229-233 bp。根据系统发育分析，分为三组。第一组包含所有黄桃，除了一个本地种间杂交中国黄桃（“白山比帽”）外，还有一些中国观赏桃和白桃。第二组分为两个子组。第1组仅包含两个中国西北地区的本地品种，而第2组中则主要包括扁桃和中国北方的本地品种。此外，还有两个相关物种，P。大卫和 P。 Ferganensis 和矮桃（ P。persica var。 densa ）聚类到第2组。第三组主要包含日本白桃品种。 P静音是唯一的，没有分配给任何组。建议桃的遗传背景为 P。大卫和 P。 ferganensis 可能是多种 P。波斯。在这项研究中，“张煌7号”，“五月仙县”和“白芒潘陶”可能是最古老的品种，而“白山比帽”是 P。大卫 x P。 persica 杂种。

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