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首页> 外文期刊>Chinese journal of digestive diseases >Oxazolone-induced murine model of ulcerative colitis.
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Oxazolone-induced murine model of ulcerative colitis.

机译：恶唑酮诱导的小鼠溃疡性结肠炎模型。

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摘要

OBJECTIVE: Animal models are useful for studying disease, but there is a shortage of suitable models of ulcerative colitis. The aim of the present study was to set up an oxazolone-induced murine colitis model and use it to research the pathogenesis of inflammatory bowel disease. METHODS: BALB/c mice were presensitized by painting the skin with 0.2 mL 3% oxazolone in 100% ethanol on days 0 and 1 followed by intrarectal administration of 0.15 mL 1% oxazolone in 50% ethanol on day 7. The disease activity index (DAI), histological changes of the colon, myeloperoxidase (MPO) activity and production of cytokines (TNF-alpha, IL-4, IFN-gamma) by the mucosa were evaluated. RESULTS: There were obvious changes in the DAI, histology and MPO activity, and the production of interleukin-4 was markedly increased compared with the concentrations of TNF-alpha and IFN-gamma, which remained normal, in the lesions. CONCLUSION: Oxazolone colitis is Th2-mediated and has similar histologic features and distribution of inflammation to ulcerative colitis (UC), which has important implications for the use of this model in the study of the pathogenesis and treatment of UC.
机译：目的：动物模型可用于研究疾病，但缺乏合适的溃疡性结肠炎模型。本研究的目的是建立恶唑酮诱导的小鼠结肠炎模型，并将其用于研究炎症性肠病的发病机理。方法：通过在第0天和第1天用0.2 mL 3％的100％乙醇中的恶唑酮粉刷皮肤，然后在第7天直肠内给药0.15 mL 1％的50％乙醇中的唑酮，对BALB / c小鼠进行预敏。 DAI），评估结肠的组织学变化，髓过氧化物酶（MPO）活性和粘膜产生的细胞因子（TNF-α，IL-4，IFN-γ）的产生。结果：病变中DAI，组织学和MPO活性均发生了明显变化，与TNF-α和IFN-γ的浓度相比，IL-4的产生明显增加。结论：恶唑酮结肠炎是Th2介导的，具有与溃疡性结肠炎（UC）相似的组织学特征和炎症分布，这对于在UC的发病机理和治疗研究中使用该模型具有重要意义。

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