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首页> 外文期刊>The Journal of hospital infection >Quantitative measurement of the efficacy of protein removal by cleaning formulations; comparative evaluation of prion-directed cleaning chemistries.
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Quantitative measurement of the efficacy of protein removal by cleaning formulations; comparative evaluation of prion-directed cleaning chemistries.

机译：定量测量清洁配方去除蛋白质的功效;病毒定向清洁化学的比较评估。

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The stability of the infectious agent causing variant Creutzfeldt-Jakob disease (vCJD) has highlighted the importance of cleaning surgical instruments for controlling potential spread of iatrogenic CJD. In this study, thermostable adenylate kinases (tAKs) in test soil were coated on to stainless steel and these surrogate agents used to evaluate the efficacy of a range of cleaning chemistries in a bench-top washer disinfector (btWD), or as a pre-soak either with or without subsequent treatment by btWD. Two tAKs were tested initially for ease of removal, the most persistent being Sulfolobus acidocaldarius-derived tAK which was used for evaluating the cleaning chemistries. Conventional chemistries were generally more effective when used in a btWD than as pre-soaks. Cleaning efficacy improved when pre-soaks were followed by treatment with intermediate performing enzymes, demonstrating greater than additive effect on residual tAK activity. Three of the four prion-directed chemistries reduced residual tAK activity to below the limit of quantification (LoQ) by more than 4.8 log(10); <175pg tAK remaining as a pre-soak alone. A conventional alkaline cleaning product also reduced residual tAK activity to below the LoQ but only when used in a btWD. tAK soil dried on to the device was removed less efficiently than tAK soil still moist on the device, with a 320-fold and 28-fold increase in residual tAK activity for pre-soak and btWD, respectively. The study demonstrated the potential for a tAK indicator to describe the effectiveness of protein removal using different chemistries or treatment processes.
机译：引起变种克雅氏病（vCJD）的传染原的稳定性凸显了清洁外科手术器械以控制医源性CJD潜在传播的重要性。在这项研究中，将测试土壤中的热稳定性腺苷酸激酶（tAKs）涂在不锈钢上，这些替代剂用于评估台式清洗消毒器（btWD）中或作为预清洗剂的一系列清洁化学的功效。浸泡在有或没有btWD后续治疗的情况下。最初测试了两种tAK的易去除性，其中最持久的是源自嗜盐球菌的tAK，用于评估清洁剂的化学性质。当用于btWD中时，常规化学方法通常比预先浸泡更有效。当预浸泡后用中等性能的酶处理后，清洁效果得到了改善，表明对残留tAK活性的影响大于累加作用。四种病毒定向化学试剂中的三种将残留的tAK活性降低到了定量限（LoQ）以下，降低了4.8 log（10）以上。 <175pg tAK仅作为预浸泡剩余。常规的碱性清洁产品还将残留的tAK活性降低到LoQ以下，但仅在btWD中使用时才如此。与干燥的tAK土壤相比，在设备上干燥的tAK土壤去除效率较低，预浸泡和btWD的残留tAK活性分别增加了320倍和28倍。这项研究证明了使用tAK指标描述使用不同化学方法或处理工艺去除蛋白质的有效性的潜力。

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