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首页> 外文期刊>The journal of microbiology >Molecular Analysis of Prokaryotic Diversity in the Deep Subsurface ofthe Former Homestake Gold Mine, South Dakota, USA
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Molecular Analysis of Prokaryotic Diversity in the Deep Subsurface ofthe Former Homestake Gold Mine, South Dakota, USA

机译：美国南达科他州前霍姆斯特克金矿深表层下原核生物多样性的分子分析

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A culture-independent molecular phylogenetic analysis was carried out to study the prokaryotic diversityin two soil samples collected from the subsurface (1.34 km depth) of the former Homestake gold mine,Lead, South Dakota, USA at two sites, the Ross shaft and number 6 Winze. Microbial community analyseswere performed by cloning and sequencing of 16S rRNA genes retrieved directly from soil samples.Geochemical characterization of soils revealed high amount of toxic metals such as As, Cd, Co, Cr, Cu,Ni, Pb, Zn, and U at both the sites. Phylogenetic analyses showed that soil samples were predominantlycomposed of phylotypes related to phylum Proteobacteria. Other phyla detected in libraries were Acidobac-teria, Actinobacteria, Bacteroidetes, Chloroflexi, Chlorobi, Firmicutes, Gemmatimonadetes, Nitrospirae, Plancto-mycetes, Verrucomicrobia, and candidate divisions OP10 and TM7. The majority (>95%) of the phylotypesretrieved in the libraries were most closely related to environmental sequences from yet-uncultured bac-teria representing a hitherto unidentified diversity. The arehaeal communities at both the sites exhibitedlower diversity and were most closely affiliated to uncultivated species within the Crenarchaeota. Resultsshowed the existence of diverse microbial populations in deep subsurface environment of the Homestakegold mine. Statistical analyses demonstrated that each site harbored phylogenetically distinct microbialpopulations that were more diverse at Ross site compare to winze site.
机译：进行了与文化无关的分子系统发育分析，研究了从前霍姆斯特克金矿（位于美国南达科他州利德）铅矿的地下（深度为1.34 km）的两个土壤样品中的原核生物多样性，这两个位置分别位于罗斯竖井和6号温兹通过对直接从土壤样品中检索到的16S rRNA基因进行克隆和测序来进行微生物群落分析。土壤的地球化学特征显示，两种土壤中都含有大量的有毒金属，例如As，Cd，Co，Cr，Cu，Ni，Pb，Zn和U网站。系统发育分析表明，土壤样品主要由与门氏杆菌相关的系统型组成。在文库中检测到的其他种系是酸杆菌属，放线菌属，拟杆菌属，绿弯曲菌，绿萝属，硬毛菌，双子叶植物，硝化螺菌属，浮游菌类，Verrucomicrobia以及候选分类OP10和TM7。文库中检索到的大多数系统型（> 95％）与来自尚未培养的细菌的环境序列最密切相关，这代表了迄今尚未发现的多样性。这两个地点的风沙群落都表现出较低的多样性，并且与Crenarchaeota内未栽培的物种密切相关。结果表明，霍姆斯塔克金矿深层地下环境中存在多种微生物种群。统计分析表明，每个站点都拥有系统发育上不同的微生物种群，与Winze站点相比，Ross站点的微生物种群更多。

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