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首页> 外文期刊>Chinese journal of digestive diseases >Effect of millimeter wave radiation on the expression of proliferating cell nuclear antigen, CDK4 and PI6 in rat hepatocellular carcinoma
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Effect of millimeter wave radiation on the expression of proliferating cell nuclear antigen, CDK4 and PI6 in rat hepatocellular carcinoma

机译：毫米波辐射对大鼠肝细胞癌中增殖细胞核抗原，CDK4和PI6表达的影响

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OBJECTIVE: To study the effects of millimeter wave (MMW) radiation on rat hepatocellular carcinoma.METHODS: Forty male Wistar rats were randomly divided into four groups, and the liver region of the rats was directly radiated by MMW (35.8 GHz, 100 mW/cm~2) for 20 min twice per week. Rats in groups 1 to 3 were fed with diethlnitrosamine (DEN). Group 1 was a tumor control group with sham radiation. Group 2 was given radiation for 10 weeks starting from week 5 and group 3 was radiated for 5 weeks starting from week 10. Group 4 was a normal control group radiated for 5 weeks and given distilled water. At week 14, all rats were sacrificed. A serologi-cal test for y-glutamyltransferase (y-GT) and immuno-histochemical staining of proliferating cell nuclear antigen (PCNA), CDK4 and PI6 in liver tissue were performed.
机译：目的：研究毫米波（MMW）辐射对大鼠肝细胞癌的影响。方法：40只雄性Wistar大鼠随机分为四组，并通过MMW（35.8 GHz，100 mW / cm〜2），每周两次，每次20分钟。第1至第3组的大鼠饲喂二亚硝基亚硝胺（DEN）。第1组是具有假放射的肿瘤对照组。从第5周开始对第2组进行辐射10周，从第10周开始对第3组进行辐射5周。第4组是正常对照组，对辐射进行5周并给予蒸馏水。在第14周，处死所有大鼠。进行了血清γ-谷氨酰转移酶（y-GT）的血清学测试以及肝组织中增殖细胞核抗原（PCNA），CDK4和PI6的免疫组织化学染色。

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