• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>The Journal of Infectious Diseases >Immunization with a tetraepitopic lipid core peptide vaccine construct induces broadly protective immune responses against group A streptococcus.
【24h】

Immunization with a tetraepitopic lipid core peptide vaccine construct induces broadly protective immune responses against group A streptococcus.

机译：用四表位脂质核心肽疫苗构建体免疫可诱导针对A组链球菌的广泛保护性免疫应答。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

BACKGROUND: The development of a vaccine to prevent infection with group A streptococcus (GAS) is hampered by the widespread diversity of circulating GAS strains and M protein types, and it is widely believed that a multivalent vaccine would provide better protective immunity. METHODS: We investigated the efficacy of incorporating 3 M protein serotypic amino-terminal epitopes from GAS isolates that are common in Australian Aboriginal communities and a conformational epitope from the conserved carboxy-terminal C-repeat region into a single synthetic lipid core peptide (LCP) vaccine construct in inducing broadly protective immune responses against GAS after parenteral delivery to mice. RESULTS: Immunization with the tetraepitopic LCP vaccine construct led to high titers of systemic, antigen-specific IgG responses and the induction of broadly protective immune responses, as was demonstrated by the ability of immune serum to opsonize multiple GAS strains. Systemic challenge of mice with a lethal dose of GAS given 60 or 300 days after primary immunization showed that, compared with the control mice, the vaccinated mice were significantly protected against GAS infection, demonstrating that the vaccination stimulated long-lasting protective immunity. CONCLUSIONS: These data support the efficacy of the LCP vaccine delivery system in the development of a synthetic, multiepitopic vaccine for the prevention of GAS infection.
机译：背景：正在流行的循环GAS菌株和M蛋白类型的多样性阻碍了预防A型链球菌（GAS）感染的疫苗的开发，并且广泛认为多价疫苗将提供更好的保护性免疫。方法：我们调查了将澳大利亚原住民社区常见的GAS分离株的3 M蛋白血清型氨基末端表位和保守的羧基末端C重复区的构象表位整合到单个合成脂质核心肽（LCP）中的功效疫苗构建物可在胃肠外递送给小鼠后诱导针对GAS的广泛保护性免疫应答。结果：用四表位LCP疫苗构建体进行免疫可导致高滴度的全身性，抗原特异性IgG反应和广泛保护性免疫反应的诱导，免疫血清能够调理多种GAS菌株的能力证明了这一点。初次免疫后60或300天给予致死剂量的GAS的小鼠全身性攻击显示，与对照小鼠相比，接种疫苗的小鼠对GAS感染具有明显的保护作用，表明疫苗接种刺激了持久的保护性免疫。结论：这些数据支持了LCP疫苗递送系统在开发合成多表位疫苗中预防GAS感染的功效。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号