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首页> 外文期刊>The Journal of Infectious Diseases >Seroepidemiology of human bocavirus defined using recombinant virus-like particles.
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Seroepidemiology of human bocavirus defined using recombinant virus-like particles.

机译：使用重组病毒样颗粒定义的人博卡病毒的血清流行病学。

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BACKGROUND: Human bocavirus (HBoV) is a newly identified human parvovirus for which seroepidemiology and antigenic properties remain undefined. METHODS: The HBoV VP2 gene, expressed from a baculovirus vector, produced virus-like particles (VLPs), which were used to raise rabbit anti-HBoV antisera and to develop an enzyme-linked immunosorbent assay (ELISA). The VLP-based ELISA was used to screen for HBoV-specific immunoglobulin G antibodies in a convenience sample of 270 serum specimens, mostly from children, obtained at Yale-New Haven Hospital; 208 specimens were also screened for erythrovirus B19-specific antibodies by a B19 VLP-based ELISA. RESULTS: Immunofluorescence and ELISA showed that human parvoviruses HBoV and B19 are antigenically distinct. By the HBoV VLP-based ELISA, 91.8% and 63.6% of serum specimens from infants in the first and second months of life, respectively, were found to be seropositive, as were 45.4% from 3-month-old infants and 25.0% from 4-month-old infants. The percentages of HBoV-seropositive children increased to 40.7%-60.0% for children 5-47 months of age and to >85% for individuals >or=48 months old. However, the overall percentage of B19-seropositive individuals was <40.5% for all age groups screened. CONCLUSIONS: HBoV infection is common during childhood, but a minority of children and young adults screened have evidence of B19 infection.
机译：背景：人类博卡病毒（HBoV）是一种新近鉴定出的人类细小病毒，其血清流行病学和抗原特性仍然不确定。方法：从杆状病毒载体表达的HBoV VP2基因产生病毒样颗粒（VLP），用于产生兔抗HBoV抗血清并开发酶联免疫吸附测定（ELISA）。基于VLP的ELISA被用于筛选从Yale-New Haven医院获得的270个血清样本的便利样本中的HBoV特异性免疫球蛋白G抗体，这些样本大多来自儿童。还通过基于B19 VLP的ELISA筛选了208个标本中的红病毒B19特异性抗体。结果：免疫荧光和酶联免疫吸附测定显示人细小病毒HBoV和B19在抗原上是不同的。通过基于HBoV VLP的ELISA，发现出生后第一个月和第二个月婴儿血清样本分别为血清阳性，分别为91.8％和63.6％，3个月大婴儿分别为45.4％和25.0％。 4个月大的婴儿。 HBoV血清阳性儿童的百分比在5-47个月大的儿童中增加到40.7％-60.0％，而对于≥48个月大的个人，增加到> 85％。但是，在所有筛查的年龄组中，B19血清阳性个体的总体百分比均低于40.5％。结论：HBoV感染在儿童时期很常见，但经筛查的少数儿童和年轻人有B19感染的证据。

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