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首页> 外文期刊>Immunology: An Official Journal of the British Society for Immunology >Immunogenicity of recombinant human bocavirus-1,2 VP2 gene virus-like particles in mice
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Immunogenicity of recombinant human bocavirus-1,2 VP2 gene virus-like particles in mice

机译：重组人博卡病毒-1，2 VP2基因病毒样颗粒在小鼠中的免疫原性

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摘要

Human bocavirus (HBoV), a recently identified pathogen with a worldwide distribution is closely related to paediatric acute respiratory infection and gastroenteritis. The present study was performed to evaluate the immunogenicity of HBoV1 and HBoV2 virus-like particles (VLPs) as vaccine candidates in mice. Both HBoV1 and HBoV2 VLPs were expressed in the bacmid virus-SF9 cell system. Mice were inoculated three times at 3-week intervals with HBoV VLPs at one dose intramuscular (i.m.) or intradermal (i.d.) with or without the addition of the alum adjuvant. ELISA was used to detected antibody, and ELISPOT was used to test cellular immune responses. HBoV-specific IgG antibodies were induced and alum adjuvant improved the antibody titres and avidity, while the inoculation pathway had no influence. T helper type 1/ type 2 immune responses were balanced induced by HBoV1 VLPs but not HBoV2 VLPs. Serum IgG antibody cross-reactivity rates of the two subtypes were similar, but cross-reactions of HBoV1 immunization groups were higher. The single i.m. group had more interferon-γ-secreting splenocytes. These data indicate that HBoV VP2 VLPs have good immunogenicity with induction of strong humoral and cellular immune responses, and they may be potential candidate vaccines for HBoV infection.
机译：人类博卡病毒（HBoV）是一种最近发现的病原，分布在世界各地，与小儿急性呼吸道感染和肠胃炎密切相关。进行本研究以评估HBoV1和HBoV2病毒样颗粒（VLP）作为小鼠候选疫苗的免疫原性。 HBoV1和HBoV2 VLP均在杆状病毒-SF9细胞系统中表达。每隔3周用一剂肌内（i.m.）或皮内（i.d.）的HBoV VLP接种小鼠三次，添加或不添加明矾佐剂。 ELISA用于检测抗体，ELISPOT用于测试细胞免疫应答。诱导了HBoV特异性IgG抗体，明矾佐剂改善了抗体滴度和亲和力，而接种途径没有影响。 T型辅助1/2型免疫应答由HBoV1 VLP而非HBoV2 VLP诱导。两种亚型的血清IgG抗体交叉反应率相似，但HBoV1免疫组的交叉反应较高。单身组有更多的γ-干扰素分泌脾细胞。这些数据表明，HBoV VP2 VLP具有良好的免疫原性，可诱导强烈的体液和细胞免疫应答，并且它们可能是HBoV感染的潜在候选疫苗。

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