• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Chromatographia >Development and validation of an HPTLC-densitometric method for determination of ACE inhibitors
【24h】

Development and validation of an HPTLC-densitometric method for determination of ACE inhibitors

机译：HPTLC密度测定法测定ACE抑制剂的开发和验证

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

A high-performance thin layer chromatographic method coupled with densitometric analysis has been developed for measurement of benazepril and cilazapril, both pure and in their commercial dosage forms. The active substances were extracted from tablets with methanol (mean recovery 102%) and chromatographed on silica gel 60 F-254 HPTLC plates in horizontal chambers with ethyl acetate-acetone-acetic acid-water, 8:2:0.5:0.5 (v/v), as mobile phase. Chromatographic separation of these ACE inhibitors was followed by UV densitometric quantitation at 215 nm. Calibration plots were constructed in the range 0.4 to 2.0 mug muL(-1) for benazepril (2.0-10.0 mug spot(-1)) and from 0.5 to 1.5 mug muL(-1) for cilazapril (4.0-12.0 mug spot(-1)) with good correlation coefficients (r greater than or equal to 0.990). The method was used to determine benazepril and cilazopril in pharmaceutical preparations with satisfactory precision (1.4% < RSD < 5.6%) and accuracy (1.7 < RE < 5.1).
机译：已开发出一种高效的薄层色谱方法，结合光密度分析法，用于测定贝那普利和西拉普利的纯品和商业剂型。用甲醇从片剂中提取活性物质（平均回收率102％），并在硅胶60 F-254 HPTLC板上在水平室内色谱分离，用乙酸乙酯-丙酮-乙酸-水（8：2：0.5：0.5（v / v），作为流动相。对这些ACE抑制剂进行色谱分离，然后在215 nm进行紫外光密度定量。苯那普利（2.0-10.0杯斑点（-1））的校准范围在0.4至2.0马克杯（-1）范围内，西拉普利（4.0-12.0马克杯）的校准范围在0.5至1.5马克杯（-1）范围内1））具有良好的相关系数（r大于或等于0.990）。该方法用于测定药物制剂中的苯那普利和西拉普利，具有令人满意的精密度（1.4％

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号