• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>The Journal of Infectious Diseases >Plasmid DNA immunization against Japanese encephalitis virus: immunogenicity of membrane-anchored and secretory envelope protein.
【24h】

Plasmid DNA immunization against Japanese encephalitis virus: immunogenicity of membrane-anchored and secretory envelope protein.

机译：针对日本脑炎病毒的质粒DNA免疫：膜锚定和分泌性包膜蛋白的免疫原性。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Plasmid DNA synthesizing membrane-anchored or secretory Japanese encephalitis virus (JEV) envelope (E) protein and premembrane protein was delivered to mice by intramuscular injection or gene gun. Intramuscular plasmid immunization induced anti-E antibody responses similar to those associated with commercial JEV vaccine. The gene gun induced less antibody response. The 2 forms of the E protein induced similar antibody titers when administered by the same delivery mode. Both plasmids generated high titers of JEV-neutralizing antibodies, although the titers were lower than those induced by the vaccine. Intramuscular DNA immunization induced T helper 1 (Th1) immune responses, and the gene gun induced Th2 responses. Compared with secretory E protein, the membrane-anchored protein heavily skewed the immune response toward either Th1 or Th2, depending on the route of immunization. In an intracerebral JEV challenge model, plasmid-immunized mice had approximately 60% protection; this was not affected by the form of the E protein or by immunization route.
机译：通过肌内注射或基因枪将合成膜锚定或分泌型日本脑炎病毒（JEV）包膜（E）蛋白和前膜蛋白的质粒DNA传递给小鼠。肌内质粒免疫诱导的抗E抗体反应类似于与商业JEV疫苗相关的反应。基因枪诱导较少的抗体反应。当以相同的递送方式给药时，两种形式的E蛋白诱导相似的抗体滴度。两种质粒均产生高滴度的JEV中和抗体，尽管滴度低于疫苗诱导的滴度。肌内DNA免疫诱导T辅助1（Th1）免疫应答，基因枪诱导Th2应答。与分泌性E蛋白相比，膜固定蛋白严重依赖于免疫途径，使免疫反应偏向Th1或Th2。在脑内JEV攻击模型中，质粒免疫的小鼠具有约60％的保护作用。这不受E蛋白形式或免疫途径的影响。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号