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首页> 外文期刊>Vaccine >Immunogenicity in mice of a cationic microparticle-adsorbed plasmid DNA encoding Japanese encephalitis virus envelope protein
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Immunogenicity in mice of a cationic microparticle-adsorbed plasmid DNA encoding Japanese encephalitis virus envelope protein

机译：阳离子微粒吸附的编码日本脑炎病毒包膜蛋白的质粒DNA在小鼠中的免疫原性

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摘要

Previously, we described a plasmid pMEa, synthesizing the anchored form of Japanese encephalitis virus (JEV) envelope protein that generated virus-neutralizing antibodies in mice upon intra-muscular injection. The immunized mice showed significant protection against lethal JEV challenge. In order to improve the efficacy of plasmid DNA immunization against JEV, methods need to be employed that would enhance neutralizing antibody titers. Plasmid DNA adsorbed to cationic microparticles has recently been shown to significantly improve its immunogenicity. In the present study, we have adsorbed the plasmid pMEa on cationic microparticles and have compared its immunogenicity with the naked plasmid DNA. As seen in ELISA, the microparticle-adsorbed DNA induced higher titers of anti-JEV antibodies when compared to those induced by the naked DNA. No difference, however, was seen in JEV neutralization titers. The microparticle-adsorbed DNA induced a mixed Th1-Th2 kind of immune responses as opposed to Th1 type of immune responses elicited by the naked DNA.
机译：以前，我们描述了质粒pMEa，它合成了日本脑炎病毒（JEV）包膜蛋白的锚定形式，该蛋白在肌肉内注射后在小鼠中产生了病毒中和抗体。免疫的小鼠显示出对致命的JEV攻击的显着保护。为了提高针对JEV的质粒DNA免疫的功效，需要采用能增强中和抗体效价的方法。最近已证明吸附在阳离子微粒上的质粒DNA可以显着提高其免疫原性。在本研究中，我们已经将质粒pMEa吸附在阳离子微粒上，并将其免疫原性与裸质粒DNA进行了比较。如ELISA中所见，与裸DNA诱导的抗体相比，微粒吸附的DNA诱导了更高的抗JEV抗体效价。然而，JEV中和效价没有差异。与裸DNA引发的Th1型免疫反应相反，微粒吸附的DNA诱导了混合的Th1-Th2类免疫反应。

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