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首页> 外文期刊>The Journal of investigative dermatology. >Changing patterns of localization of putative stem cells in developing human hair follicles.
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Changing patterns of localization of putative stem cells in developing human hair follicles.

机译：发育中的人类毛囊中假定的干细胞定位的变化模式。

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In rodents, the hair follicle stem cells lie in a well-defined bulge in the outer root sheath; however, the bulge as a stem cell site of human hair follicle epithelium is still controversial. Epidermal stem cells are thought to express high levels of beta1 integrin and low levels of E-cadherin and beta- and gamma-catenin. In order to clarify the ontogenic distribution of possible stem cells during hair follicle development, the expression patterns of beta1 integrin subunits, E-cadherin, and beta- and gamma-catenins in the skin samples from human fetuses of a series of estimated gestational ages (EGA) were examined. beta1 integrin-rich, E-cadherin-, and beta- and gamma-catenin-poor cells, possible stem cells, were localized to the entire hair germ (65-84 d EGA) and later to the outermost cells of hair peg (85-104 d EGA). In the bulbous hair peg (105-135 d EGA) and in the differentiated lanugo hair follicle (>135 d EGA), they were settled in the bulge and the outermost layer of the outer root sheath. This sequential localization was similar to that of cells rich in epidermal growth factor receptor expression and positive with keratin 19, a putative marker of epidermal stem cells. In addition, these beta1 integrin-rich, E-cadherin-, and beta- and gamma-catenin-poor cells showed similar, undifferentiated morphologic features by electron microscopy. This information of ontogenic localization of possible hair follicle stem cells contributes to the further understanding of mechanisms of human hair follicle morphogenesis and supports the idea that the human fetal hair follicle bulge is a site of stem cells for follicular epithelium.
机译：在啮齿动物中，毛囊干细胞位于外根鞘的一个明确的凸起中。然而，作为人类毛囊上皮干细胞位点的隆突仍存在争议。人们认为表皮干细胞表达高水平的β1整合素和低水平的E-钙黏着蛋白以及β-和γ-连环蛋白。为了阐明毛囊发育过程中可能的干细胞的本体分布，β1整联蛋白亚基，E-钙粘蛋白以及β-和γ-连环蛋白在一系列估计胎龄的人胎儿皮肤样品中的表达模式（ EGA）。富含beta1整联蛋白，缺乏E-钙粘着蛋白，缺乏β-和γ-连环蛋白的细胞（可能是干细胞）位于整个发芽区（65-84 d EGA），后来位于发夹的最外层细胞（85） -104 d EGA）。在球状发夹（105-135 d EGA）和分化的胎毛毛囊（> 135 d EGA）中，它们定居在外根鞘的隆起和最外层。这种顺序的定位类似于富含表皮生长因子受体表达并且角蛋白19（表皮干细胞的公认标志物）呈阳性的细胞。此外，通过电子显微镜观察，这些富含β1整合素的E-钙黏着蛋白，缺乏β-和γ-连环蛋白的细胞显示出相似的，未分化的形态特征。有关可能的毛囊干细胞的本体定位的信息有助于进一步理解人毛囊形态发生的机制，并支持人胎儿毛囊隆起是毛囊上皮干细胞的位点的观点。

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