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首页> 外文期刊>The Journal of investigative dermatology. >alpha-Melanocyte-Stimulating Hormone, MSH 11-13 KPV and Adrenocorticotropic Hormone Signalling in Human Keratinocyte Cells.
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alpha-Melanocyte-Stimulating Hormone, MSH 11-13 KPV and Adrenocorticotropic Hormone Signalling in Human Keratinocyte Cells.

机译：人角质形成细胞中的α-刺激黑素细胞激素，MSH 11-13 KPV和促肾上腺皮质激素信号传导。

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摘要

alpha-MSH signals by binding to the melanocortin-1 receptor (MC-1R) and elevating cyclic AMP in several different cells. The anti-inflammatory properties of this peptide are also believed to be cyclic AMP dependent. The carboxyl terminal tripeptides of alpha-MSH (KPV / KP-D-V) are the smallest minimal sequences reported to prevent inflammation but it is not known if they operate via MC-1R or cyclic AMP. The aim of this study was to examine the intracellular signalling of key MSH and ACTH peptides in human keratinotocytes. No elevation in cyclic AMP was detected in either HaCaT or normal human keratinocytes in response to alpha-MSH, KPV or ACTH peptides. Rapid and acute intracellular calcium, however, were observed in HaCaT keratinocytes in response to alpha-MSH (10(-15)-10(-7) M), KPV (10(-15)-10(-7) M), KP-D-V (10(-15)-10(-7) M) and ACTH (10(-15)-10(-7) M), but only in the presence of PIA, an adenosine agonist that inhibits the cyclic AMP pathway. Normal keratinocytes responded to all the above peptides but in addition responded to ACTH 1-17 (10(-13)-10(-7) M) in contrast to the HaCaT keratinocytes. Stable transfection of Chinese hamster ovary cells with the MC-1 receptor showed that alpha-MSH and the KPV peptides elevated intracellular calcium.
机译：α-MSH通过与黑皮质素1受体（MC-1R）结合并在几个不同的细胞中升高环状AMP发出信号。该肽的抗炎特性也被认为是环AMP依赖性的。据报道，α-MSH（KPV / KP-D-V）的羧基末端三肽是预防炎症的最小最小序列，但尚不清楚它们是否通过MC-1R或环状AMP运作。这项研究的目的是检查人角质形成细胞中关键MSH和ACTH肽的细胞内信号传导。响应α-MSH，KPV或ACTH肽，在HaCaT或正常人角质形成细胞中均未检测到环状AMP的升高。然而，在HaCaT角质形成细胞中，对α-MSH（10（-15）-10（-7）M），KPV（10（-15）-10（-7）M）的反应中观察到了快速和急性的细胞内钙。 KP-DV（10（-15）-10（-7）M）和ACTH（10（-15）-10（-7）M），但仅在存在PIA的情况下才能抑制环AMP的腺苷激动剂途径。正常的角质形成细胞对上述所有肽均产生反应，但与HaCaT角质形成细胞相比，其对ACTH 1-17（10（-13）-10（-7）M）也产生响应。 MC-1受体稳定转染中国仓鼠卵巢细胞表明，α-MSH和KPV肽可增加细胞内钙。

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