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首页> 外文期刊>The Journal of investigative dermatology. >A Chemiluminescence Study of UVA-Induced Oxidative Stress in Human Skin In Vivo.
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A Chemiluminescence Study of UVA-Induced Oxidative Stress in Human Skin In Vivo.

机译：人体皮肤中UVA诱导的氧化应激的化学发光研究。

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Oxidative stress is defined as an imbalance between pro-oxidants and antioxidants in favor of pro-oxidants. Photon emission (also called chemiluminescence) has been widely used to study oxidative stress in biological systems in vitro. In vivo chemiluminescence has been proposed as a non-invasive method to assess oxidative stress in the skin. UVA (320-400 nm part of the ultraviolet radiation) exposure is generally accepted as a source of oxidative stress in the skin. In this study, UVA-induced oxidative stress was studied by using an in vivo chemiluminescence detection method. First, the dose response and the fluence rate response of the UVA-induced oxidative stress in human skin were investigated by examining the decay kinetics of the chemiluminescence signal following UVA exposure. A kinetic model was proposed to help differentiate these two responses. We found that the initial burst of the chemiluminescence signal depended on the UVA fluence rate, whereas the decay of the signal following exposure can be related to the UVA dose involved. Second, a significant reduction of UVA-induced chemiluminescence signal was observed after tape-stripping, indicating that stratum corneum is a major source of UVA-induced oxidative stress in the skin. Furthermore, the oxygen dependence of UVA-induced chemiluminescence signal was also confirmed by application of a pressure cuff, implying that some of the oxidative stress occurs in the deeper layers of the skin. Finally, topical application of vitamin C before exposure significantly reduced the UVA-induced chemiluminescence signal. We thus conclude that chemiluminescence is an effective method to assess the oxidative stress induced by UVA in human skin in vivo.
机译：氧化应激定义为促氧化剂和抗氧化剂之间的失衡，有利于促氧化剂。光子发射（也称为化学发光）已被广泛用于研究体外生物系统中的氧化应激。体内化学发光已被提议作为评估皮肤中氧化应激的非侵入性方法。 UVA（紫外线辐射的320-400 nm部分）暴露通常被认为是皮肤中氧化应激的来源。在这项研究中，通过使用体内化学发光检测方法研究了UVA诱导的氧化应激。首先，通过检查UVA暴露后化学发光信号的衰减动力学，研究了UVA诱导的人体皮肤氧化应激的剂量响应和注量速率响应。提出了动力学模型来帮助区分这两种反应。我们发现化学发光信号的初始爆发取决于UVA的通量率，而暴露后信号的衰减可能与所涉及的UVA剂量有关。第二，剥离胶带后观察到UVA诱导的化学发光信号显着降低，这表明角质层是UVA诱导的皮肤氧化应激的主要来源。此外，通过施加压力袖带也证实了UVA诱导的化学发光信号的氧依赖性，这意味着某些氧化应激发生在皮肤的较深层中。最后，在暴露前局部应用维生素C可显着降低UVA诱导的化学发光信号。因此，我们得出结论，化学发光是一种有效的方法，可以评估体内人皮肤中UVA诱导的氧化应激。

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