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首页> 外文期刊>The Journal of investigative dermatology. >Highly complex peptide aggregates of the S100 fused-type protein hornerin are present in human skin.
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Highly complex peptide aggregates of the S100 fused-type protein hornerin are present in human skin.

机译：S100融合型蛋白hornerin的高度复杂的肽聚集体存在于人体皮肤中。

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摘要

Human hornerin (HRNR) is a 245 kDa S100 fused-type protein which contains 95% tandem quasi-repeating glycine- and serine-rich domains. Previously HRNR was not thought to be expressed in healthy skin; however, we purified an HRNR peptide fragment from stratum corneum. Moreover, we found that HRNR mRNA is expressed in skin biopsies from different sites as head, trunk, legs, hands, and feet. In cultured human epidermal keratinocytes, HRNR mRNA expression was transiently induced during Ca(2+)-dependent differentiation. Immunostaining using distinct antibodies generated against four putative HRNR domains revealed strong HRNR immunoreactivity in healthy epidermis as well as in the entire outer root sheath of normal human scalp hair follicles. In lesions from psoriasis and atopic dermatitis patients, HRNR immunoreactivity was reduced compared with uninvolved skin of these patients. Electrospray ionization mass spectrometry and Western blot analyses revealed that HRNR is a highly degradable protein that forms complex high molecular weight peptide aggregates. Our findings suggest that HRNR is expressed in healthy skin and give insight into the complex biology of this protein. HRNR and its degradation products might contribute to the barrier function of healthy human skin.
机译：人角蛋白（HRNR）是一种245 kDa S100融合型蛋白质，其中包含95％串联的准重复富甘氨酸和丝氨酸结构域。以前不认为HRNR在健康的皮肤中表达。但是，我们从角质层纯化了HRNR肽片段。此外，我们发现HRNR mRNA在不同部位的皮肤活检组织中表达，如头，躯干，腿，手和脚。在培养的人表皮角质形成细胞中，HRNR mRNA表达在Ca（2+）依赖性分化过程中被短暂诱导。使用针对四个推定的HRNR域生成的独特抗体进行的免疫染色显示，在健康的表皮以及正常人头皮毛囊的整个外根鞘中，HRNR都具有很强的免疫反应性。在牛皮癣和特应性皮炎患者的病变中，与这些患者的未感染皮肤相比，HRNR免疫反应性降低。电喷雾电离质谱和蛋白质印迹分析表明，HRNR是一种高度可降解的蛋白质，可形成复杂的高分子量肽聚集体。我们的研究结果表明HRNR在健康的皮肤中表达，并深入了解这种蛋白质的复杂生物学。 HRNR及其降解产物可能有助于健康人类皮肤的屏障功能。

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