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首页> 外文期刊>The Journal of laboratory and clinical medicine >Expression and function of Fas and Fas ligand on peripheral blood lymphocytes in normal subjects.
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Expression and function of Fas and Fas ligand on peripheral blood lymphocytes in normal subjects.

机译：正常受试者外周血淋巴细胞中Fas和Fas配体的表达和功能。

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We investigated the expression and function of Fas and Fas ligand (FasL) on peripheral blood lymphocytes (PBLs). The cells were stimulated with various cytokines or 12-0-tetradecanoyl phorbol 13-acetate (PMA) plus ionomycin. About 30% of unstimulated PBLs expressed Fas, and the expression was augmented by interleukin-1beta (IL-1beta), IL-2, tumor necrosis factor-alpha (TNF-alpha), interferon-gamma (IFN-gamma), or PMA plus ionomycin. Although only minimal FasL expression was detected on unstimulated PBLs, FasL expression was markedly induced by IL-2 or PMA plus ionomycin, suggesting that Fas and FasL were both expressed on IL-2-stimulated or PMA-plus-ionomycin-stimulated PBLs. Although IL-2-stimulated or PMA-plus-ionomycin-stimulated PBLs were positive for both Fas and FasL, no significant increase in apoptosis was demonstrated in these activated PBLs. In addition, treatment of PBLs with IL-2 or PMA plus ionomycin did not change anti-Fas-induced apoptosis, although these activated PBLs expressed Fas strongly when compared with unstimulated PBLs. Only IL-2-stimulated or PMA-plus-ionomycin-stimulated PBLs killed Fas+ target cells efficiently via the interaction of Fas on target cells with FasL of PBLs. Bcl-2 was constitutively expressed on unstimulated PBLs, but its expression was significantly augmented by IL-2 or PMA plus ionomycin. The expression of Bax was clearly induced only on IL-2-stimulated or PMA-plus-ionomycin-stimulated PBLs and that of other Bcl-2 family proteins such as Bcl-x and Bad could not be detected on human PBLs, including IL-2-stimulated or PMA-plus-ionomycin-stimulated PBLs. Our results suggest that PBLs activated by IL-2 or PMA plus ionomycin express both Fas and FasL and that they kill Fas+ target cells by using FasL on the surface. The resistance of these activated PBLs to Fas-mediated apoptosis may be due to the augmented Bcl-2 expression or the presence of Bcl-2:Bax heterodimers on these cells.
机译：我们调查了Fas和Fas配体（FasL）在外周血淋巴细胞（PBLs）上的表达和功能。用各种细胞因子或12-0-十四烷酰佛波醇13-乙酸盐（PMA）加离子霉素刺激细胞。约30％的未刺激PBLs表达Fas，并且白介素-1β（IL-1beta），IL-2，肿瘤坏死因子-α（TNF-alpha），干扰素-γ（IFN-γ）或PMA增强了表达加离子霉素。尽管在未刺激的PBL上仅检测到最小的FasL表达，但IL-2或PMA加离子霉素可明显诱导FasL表达，这表明Fas和FasL均在IL-2刺激或PMA加离子霉素刺激的PBLs上表达。尽管IL-2刺激的或PMA加离子霉素刺激的PBL对Fas和FasL均为阳性，但在这些活化的PBL中，凋亡没有明显增加。此外，用IL-2或PMA加离子霉素处理PBL不会改变抗Fas诱导的凋亡，尽管与未刺激的PBL相比，这些活化的PBL强烈表达Fas。仅IL-2刺激的或PMA加离子霉素刺激的PBL通过靶细胞上的Fas与PBL的FasL相互作用有效杀死Fas +靶细胞。 Bcl-2在未刺激的PBLs上组成性表达，但IL-2或PMA加离子霉素显着增强了Bcl-2的表达。显然只有在IL-2刺激或PMA加离子霉素刺激的PBLs上诱导了Bax的表达，而在人类PBLs上未检测到其他Bcl-2家族蛋白如Bcl-x和Bad的表达，包括IL- 2刺激或PMA加离子霉素刺激的PBL。我们的结果表明，被IL-2或PMA加离子霉素激活的PBL既表达Fas又表达FasL，并且它们通过在表面上使用FasL杀死Fas +靶细胞。这些活化的PBL对Fas介导的细胞凋亡的抗性可能是由于这些细胞上Bcl-2表达的增强或Bcl-2：Bax异二聚体的存在。

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