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首页> 外文期刊>The Journal of Neuroscience: The Official Journal of the Society for Neuroscience >PSD-95 is associated with the postsynaptic density and not with the presynaptic membrane at forebrain synapses.
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PSD-95 is associated with the postsynaptic density and not with the presynaptic membrane at forebrain synapses.

机译：PSD-95与前脑突触中的突触后密度相关，与突触前膜无关。

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摘要

PSD-95, a prominent protein component of the postsynaptic density (PSD) fraction from rat forebrain, has been localized by light microscopy to dendrites of hippocampal neurons (Cho et al., 1992) and to the presynaptic plexus of cerebellar basket cells (Kistner et al., 1993). Here we extend these studies to show that an affinity-purified antibody to PSD-95 labels the dendrites of most neurons in the forebrain and of a subset of neurons in the cerebellum. To confirm that PSD-95 is associated with the PSD at forebrain synapses and to clarify whether it is also associated with the presynaptic membrane, we employed immunogold electron microscopy of forebrain synaptosomes. Gold-labeled antibodies to PSD-95 labeled postsynaptic densities in both intact and lysed forebrain synaptosomes but did not label presynaptic terminals or the presynaptic membrane. The asymmetric distribution of PSD-95 at synapses contrasts with that of its homologs, disks-large and ZO-1, which are arranged symmetrically at septate and tight junctions, respectively.
机译：PSD-95是大鼠前脑突触后密度（PSD）组分的重要蛋白质成分，通过光学显微镜已定位于海马神经元的树突（Cho等，1992）和小脑篮细胞的突触前神经丛（Kistner）等人，1993）。在这里，我们扩展了这些研究，以显示针对PSD-95的亲和纯化抗体可标记前脑中大多数神经元和小脑中一部分神经元的树突。为了确认PSD-95与前脑突触处的PSD相关并澄清它是否也与突触前膜相关，我们采用了前脑突触体的免疫金电子显微镜技术。金标记的PSD-95抗体在完整和裂解的前脑突触小体中标记了突触后密度，但未标记突触前末端或突触前膜。 PSD-95在突触处的不对称分布与其同系物，大盘状和ZO-1的分布相反，后者分别在分隔的连接处和紧密的连接处对称排列。

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