• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>The Journal of Neuroscience: The Official Journal of the Society for Neuroscience >Activation by serotonin and noradrenaline of vasopressin and oxytocin expression in the mouse paraventricular and supraoptic nuclei.
【24h】

Activation by serotonin and noradrenaline of vasopressin and oxytocin expression in the mouse paraventricular and supraoptic nuclei.

机译：5-羟色胺和去甲肾上腺素激活小鼠心室旁和视上核中血管加压素和催产素的表达。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Noradrenaline and serotonin are known to control arginine-vasopressin (AVP) and oxytocin (OT) secretion in the systemic circulation. The aim of the current study was to investigate whether these monoamines are also able to influence AVP and OT expression in the paraventricular (PVN) and supraoptic nuclei (SON). To test this hypothesis, we used the Tg8 transgenic mice KO for the monoamine oxidase-A gene, which present high levels of noradrenaline and serotonin in the brain. AVP and OT expression were evaluated at peptide and mRNA levels by immunohistochemistry, enzyme immunoassay, and in situ hybridization. Compared with wild type, the amounts of AVP, OT, AVP mRNA, and OT mRNA were increased in the PVN and SON in Tg8 mice. To distinguish the respective contributions of noradrenaline and serotonin to these modifications, we treated Tg8 mice with a synthesis inhibitor of either catecholamines [alpha-methylparatyrosine (alpha-MPT)] or serotonin [parachlorophenylalanine (pCPA)]. Administration of alpha-MPT to Tg8 mice induced a decline in the amounts of AVP, OT, and their mRNA in the PVN and SON. The pCPA treatment in Tg8 mice was also associated with a decrease in OT expression in the PVN and SON and in AVP expression in the PVN, but not in the SON. These results suggest that noradrenaline may activate AVP and OT expression in the PVN and SON. Likewise, serotonin is proposed to stimulate AVP and OT expression in the PVN and only OT expression in the SON.
机译：已知去甲肾上腺素和5-羟色胺可控制体循环中的精氨酸加压素（AVP）和催产素（OT）分泌。本研究的目的是研究这些单胺是否也能影响心室旁（PVN）和视上核（SON）中的AVP和OT表达。为了验证这一假设，我们使用了Tg8转基因小鼠KO的单胺氧化酶A基因，该基因在大脑中存在高水平的去甲肾上腺素和血清素。通过免疫组织化学，酶免疫测定和原位杂交在肽和mRNA水平评估AVP和OT表达。与野生型相比，Tg8小鼠的PVN和SON中AVP，OT，AVP mRNA和OT mRNA的含量增加。为了区分去甲肾上腺素和5-羟色胺对这些修饰的各自贡献，我们用儿茶酚胺[α-甲基对酪氨酸（α-MPT）]或5-羟色胺[对氯苯丙氨酸（pCPA）]的合成抑制剂处理了Tg8小鼠。给予Tg8小鼠alpha-MPT导致PVN和SON中AVP，OT及其mRNA含量下降。 Tg8小鼠中的pCPA治疗还与PVN和SON中的OT表达下降以及PVN中的AVP表达下降有关，但与SON中的下降无关。这些结果表明去甲肾上腺素可能激活PVN和SON中的AVP和OT表达。同样，提出了5-羟色胺刺激PVN中的AVP和OT表达，而仅刺激SON中的OT表达。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号