• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>The Journal of Neuroscience: The Official Journal of the Society for Neuroscience >Tissue- and age-specific expression patterns of alternatively spliced agrin mRNA transcripts in embryonic rat suggest novel developmental roles.
【24h】

Tissue- and age-specific expression patterns of alternatively spliced agrin mRNA transcripts in embryonic rat suggest novel developmental roles.

机译：胚胎大鼠中选择性剪接的凝集素mRNA转录物的组织和年龄特异性表达模式提示新的发育作用。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Agrin is an extracellular matrix protein that mediates the nerve-induced clustering of nicotinic acetylcholine receptors on target muscle cells, and thus plays a key role in development of the neuromuscular synapse. Alternative exon usage within the rat agrin gene predicts numerous protein isoforms, which differ by the inclusion or exclusion of small inserts at three sites in the C-terminal half of the molecule; the insert status at two of these sites, termed Y and Z, profoundly influences the acetylcholine receptor clustering activity. We have examined the cellular expression patterns of agrin messenger RNA transcripts during rat embryogenesis by in situ hybridization with isoform-specific probes. Six 36-mer oligonucleotide probes were designed to distinguish between mRNA isoforms at either the Y site: the encoded protein contains either no insert (Y0) or a 4-amino acid insert (Y4), or the Z site: the encoded protein contains either no insert or one of 8 (Z8), 11 (Z11), or 19 (Z19) amino acids. Strikingly different expression patterns were observed for the individual Y- and Z-site encoding messages. While optional exon usage predicts the possibility of eight different agrin isoforms at the two splice sites, we detected only four isoforms in vivo: Y4Z0, Y0Z0, Y4Z8, and Y4Z19. The Y4Z0 transcript, which comprised the majority of the agrin expressed, was localized exclusively to nervous tissue and exhibited a distribution profile suggestive of a potential role in neurogenesis and/or neural differentiation. From embryonic day 13 to birth, Y4Z0 was found in mitotic ventricular zones, spinal, cranial, and sympathetic ganglia, and diffusely throughout the brain. In contrast, Y0Z0 was not expressed in neurons, but specifically labeled capillary endothelial cells within the developing nervous system. Y4Z8 and Y4Z19, the forms most active in acetylcholine receptor aggregation, were expressed at low levels only in spinal and brainstem motor neurons; Z19 expression declined from embryonic day 15 to adulthood, whereas Z8 expression increased slightly during this period. Transcripts encoding the Z11 insert could not be detected. These data suggest potential novel biological roles for agrin beyond that originally proposed in synapse formation.
机译：Agrin是一种细胞外基质蛋白，可介导神经诱导的烟碱乙酰胆碱受体在靶肌细胞上的聚集，因此在神经肌肉突触的发育中起关键作用。大鼠凝集素基因中外显子的替代用法可预测多种蛋白质同工型，其不同之处在于在分子C端一半的三个位点包含或不包含小插入片段;这些位点中的两个的插入状态（称为Y和Z）会深刻影响乙酰胆碱受体的聚类活性。我们已经通过与同工型特异性探针原位杂交检查了大鼠胚胎发生过程中凝集素信使RNA转录本的细胞表达模式。设计了六种36-mer寡核苷酸探针，以区分Y位点的mRNA同工型：编码的蛋白不包含插入物（Y0）或4-氨基酸插入物（Y4）或Z位点：编码的蛋白包含任一个没有插入物或8（Z8），11（Z11）或19（Z19）个氨基酸之一。对于单独的Y位和Z位编码消息，观察到了惊人的不同表达模式。虽然可选的外显子用法预测了在两个剪接位点可能存在八种不同的凝集素同工型的可能性，但我们在体内仅检测到四种同工型：Y4Z0，Y0Z0，Y4Z8和Y4Z19。包含大部分表达的凝集​​素的Y4Z0转录本仅定位于神经组织，并显示出分布图，暗示了在神经发生和/或神经分化中的潜在作用。从胚胎的第13天到出生，Y4Z0被发现在有丝分裂的心室区，脊髓，颅骨和交感神经节中，并在整个大脑中扩散。相反，Y0Z0不是在神经元中表达，而是在发育中的神经系统内被特异性标记的毛细血管内皮细胞。 Y4Z8和Y4Z19是乙酰胆碱受体聚集中最活跃的形式，仅在脊髓和脑干运动神经元中低水平表达。从胚胎第15天到成年，Z19表达下降，而在此期间Z8表达略有增加。无法检测到编码Z11插入物的转录本。这些数据表明，除了最初在突触形成中提出的作用以外，凝集素的潜在新的生物学作用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号