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A rapid high-performance liquid chromatographic method for quantitative analysis of antidiabetic-active components in Anemarrhena asphodeloides rhizomes

机译:快速高效液相色谱法定量分析知母知母中抗糖尿病活性成分

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摘要

Extracts of herbs with promising antidiabetic capacity, used in traditional medicine, have been analyzed by high-performance liquid chromatography (HPLC) coupled with diode-array detection (DAD). Optimization of the chromatographic conditions is discussed. Two kinds of xanthone glycoside were successfully separated and quantified in herbs from seven regions. The neomangiferin and mangiferin content of herbs from different regions varied greatly. The sensitivity, selectivity, accuracy, precision, and ruggedness of the proposed method were excellent, and the method can be used for quality control of Anemarrhena asphodeloides rhizome raw material.
机译:通过高效液相色谱(HPLC)与二极管阵列检测(DAD)结合,对传统医学中具有良好抗糖尿病能力的草药提取物进行了分析。讨论了色谱条件的优化。从七个地区的草药中成功分离并定量了两种x吨酮苷。不同地区草药的新芒果苷和芒果苷含量差异很大。所提方法的灵敏度,选择性,准确性,精密度和耐用性均极好,可用于知母知母原料的质量控制。

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