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Demonstration of high speeds with low pressure drops using 1.8 μm particles in SFC

机译：在SFC中使用1.8μm颗粒进行低压降的高速演示

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The literature contains few, if any, references to the use of sub-2 μm particles in supercritical fluid chromatography (SFC). In this study, the use of 1.8 μm particles in SFC is demonstrated, producing high efficiency chromatograms, less than 1 min long for each of a diverse range of solute families, including steroids, sulfonamides, profens, nucleic acids and xanthenes. Most of the solutes eluted from bare silica with surprisingly good peak shapes. The 3 × 100 mm column packed with 1.8 μm spherical silica particles, produced as many as 22,400 plates (80% of theoretical). Column head pressure did not exceed 410 bar and pressure drops did not exceed 240 bar, even at flows of 3 mL min~(-1), or modifier concentration as high as 65%. Such performance produced the fastest solvation based chromatography (LC-SFC) reported to date. The chromatographic hardware requirements are met by older (400 bar max.) LC equipment. The speeds observed exceed even ultra high performance liquid chromatography (UHPLC), which requires dramatically higher pressure capability. Sub 2-μm particles appear suitable for routine use, employing common 400 bar LC equipment, only slightly modified to perform SFC, although faster detectors and smaller flow cells enhance performance.
机译：文献中几乎没有提及超临界流体色谱（SFC）中亚2μm颗粒的使用。在这项研究中，已证明在SFC中使用了1.8μm的颗粒，可产生高效色谱图，对于各种溶质家族（包括类固醇，磺酰胺，脯氨酸，核酸和黄嘌呤），每种色谱柱的长度都不到1分钟。从裸露的二氧化硅洗脱的大多数溶质具有出乎意料的良好峰形。装有1.8μm球形二氧化硅颗粒的3×100 mm色谱柱，可生产多达22,400块板（理论值的80％）。即使在3 mL min〜（-1）的流量或改性剂浓度高达65％的情况下，柱头压力也不会超过410 bar，压降也不会超过240 bar。这种性能产生了迄今为止报道的最快的基于溶剂化的色谱法（LC-SFC）。较旧的（最大400 bar）LC设备可以满足色谱硬件的要求。观察到的速度甚至超过了超高效液相色谱（UHPLC），后者需要显着更高的承压能力。使用普通的400 bar液相色谱设备，亚2微米以下的颗粒似乎适合常规使用，只是稍加修改即可进行SFC，尽管更快的检测器和更小的流通池可提高性能。

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